Literature DB >> 6329694

Regulated high efficiency expression of human interferon-alpha in Saccharomyces cerevisiae.

M F Tuite, M J Dobson, N A Roberts, R M King, D C Burke, S M Kingsman, A J Kingsman.   

Abstract

The 5' control region of the yeast phosphoglycerate kinase gene (PGK) was fused to the coding sequence of a human interferon-alpha. This PGK-interferon fusion was then introduced into yeast on a high copy number 2mu-based plasmid vector. Strains containing this plasmid produced a PGK-interferon-alpha fusion protein as 1-2% of cell protein and the expression of interferon activity was regulated by the availability of a fermentable carbon source. The system is capable of making as much as 15 mg of human interferon-alpha per litre of batch culture.

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Year:  1982        PMID： 6329694      PMCID： PMC553094          DOI： 10.1002/j.1460-2075.1982.tb01215.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  28 in total

1.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

2.  Effect of glucose on the level of glycolytic enzymes in yeast.

Authors:  F A Hommes
Journal:  Arch Biochem Biophys       Date:  1966-04       Impact factor: 4.013

3.  Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.

Authors:  M Kozak
Journal:  Nucleic Acids Res       Date:  1981-10-24       Impact factor: 16.971

4.  Interferon induction by viruses and polynucleotides: a differential effect of comptothecin.

Authors:  K T Atherton; D C Burke
Journal:  J Gen Virol       Date:  1975-12       Impact factor: 3.891

5.  Overlap hybridization screening: isolation and characterization of overlapping DNA fragments surrounding the leu2 gene on yeast chromosome III.

Authors:  A C Chinault; J Carbon
Journal:  Gene       Date:  1979-02       Impact factor: 3.688

6.  High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

Authors:  K Struhl; D T Stinchcomb; S Scherer; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

7.  DNA sequence required for efficient transcription termination in yeast.

Authors:  K S Zaret; F Sherman
Journal:  Cell       Date:  1982-03       Impact factor: 41.582

8.  Human leukocyte interferon produced by E. coli is biologically active.

Authors:  D V Goeddel; E Yelverton; A Ullrich; H L Heyneker; G Miozzari; W Holmes; P H Seeburg; T Dull; L May; N Stebbing; R Crea; S Maeda; R McCandliss; A Sloma; J M Tabor; M Gross; P C Familletti; S Pestka
Journal:  Nature       Date:  1980-10-02       Impact factor: 49.962

9.  Mechanism of interferon action: simian virus 40-specific early polypeptides synthesized in untreated and interferon-treated monkey kidney cells.

Authors:  S M Kingsman; M D Smith; C E Samuel
Journal:  Proc Natl Acad Sci U S A       Date:  1980-05       Impact factor: 11.205

10.  Faithful and efficient translation of homologous and heterologous mRNAs in an mRNA-dependent cell-free system from Saccharomyces cerevisiae.

Authors:  M F Tuite; J Plesset; K Moldave; C S McLaughlin
Journal:  J Biol Chem       Date:  1980-09-25       Impact factor: 5.157
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  43 in total

1.  Characterization of the transcriptional potency of sub-elements of the UAS of the yeast PGK gene in a PGK mini-promoter.

Authors:  C A Stanway; A Chambers; A J Kingsman; S M Kingsman
Journal:  Nucleic Acids Res       Date:  1989-11-25       Impact factor: 16.971

2.  Codon replacement in the PGK1 gene of Saccharomyces cerevisiae: experimental approach to study the role of biased codon usage in gene expression.

Authors:  A Hoekema; R A Kastelein; M Vasser; H A de Boer
Journal:  Mol Cell Biol       Date:  1987-08       Impact factor: 4.272

3.  Construction of a regulated PGK expression vector.

Authors:  D J Cousens; M J Wilson; E Hinchliffe
Journal:  Nucleic Acids Res       Date:  1990-03-11       Impact factor: 16.971

4.  Extensive mutagenesis of a transcriptional activation domain identifies single hydrophobic and acidic amino acids important for activation in vivo.

Authors:  M B Sainz; S A Goff; V L Chandler
Journal:  Mol Cell Biol       Date:  1997-01       Impact factor: 4.272

5.  Multiple cooperative interactions constrain BPV-1 E2 dependent activation of transcription.

Authors:  M Sowden; S Harrison; R Ashfield; A J Kingsman; S M Kingsman
Journal:  Nucleic Acids Res       Date:  1989-04-25       Impact factor: 16.971

6.  Multiple control elements in the TRP1 promoter of Saccharomyces cerevisiae.

Authors:  S Kim; J Mellor; A J Kingsman; S M Kingsman
Journal:  Mol Cell Biol       Date:  1986-12       Impact factor: 4.272

7.  A transcriptional activator is located in the coding region of the yeast PGK gene.

Authors:  J Mellor; M J Dobson; A J Kingsman; S M Kingsman
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

Review 8.  The yeast expression system for recombinant glycosyltransferases.

Authors:  M Malissard; S Zeng; E G Berger
Journal:  Glycoconj J       Date:  1999-02       Impact factor: 2.916

9.  Regulated expression of a human interferon gene in yeast: control by phosphate concentration or temperature.

Authors:  R A Kramer; T M DeChiara; M D Schaber; S Hilliker
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205

10.  Yeast glycolytic mRNAs are differentially regulated.

Authors:  P A Moore; F A Sagliocco; R M Wood; A J Brown
Journal:  Mol Cell Biol       Date:  1991-10       Impact factor: 4.272
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