| Literature DB >> 6159538 |
D V Goeddel, E Yelverton, A Ullrich, H L Heyneker, G Miozzari, W Holmes, P H Seeburg, T Dull, L May, N Stebbing, R Crea, S Maeda, R McCandliss, A Sloma, J M Tabor, M Gross, P C Familletti, S Pestka.
Abstract
A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli. The DNA sequence codes for a 23-amino acid signal peptide followed by an interferon polypeptide of 165 amino acids. An expression plasmid was constructed which permits the synthesis in E. coli of 2.5 x 10(8) units of interferon per litre of culture. This LeIF protected squirrel monkeys from lethal encephalomyocarditis virus infection.Entities:
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Year: 1980 PMID： 6159538 DOI： 10.1038/287411a0
Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962