Literature DB >> 6320183

Regulated expression of a human interferon gene in yeast: control by phosphate concentration or temperature.

R A Kramer, T M DeChiara, M D Schaber, S Hilliker.   

Abstract

The promoter/regulator region from the yeast repressible acid phosphatase gene was used to construct a vector for the regulated expression of cloned genes in yeast. The gene for human leukocyte interferon was inserted into this vector. Yeast cells transformed with the resulting plasmid produced significant amounts of interferon only when grown in medium lacking inorganic phosphate. Mutants in two acid phosphatase regulatory genes (coding for a defective repressor and a temperature-sensitive positive regulator) were used to develop a yeast strain that grew well at a high temperature (35 degrees C) but produced interferon only at a low temperature (23 degrees C), independent of phosphate concentration.

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Year:  1984        PMID： 6320183      PMCID： PMC344677          DOI： 10.1073/pnas.81.2.367

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  22 in total

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Authors:  Y Ueda; A To-E; Y Oshima
Journal:  J Bacteriol       Date:  1975-06       Impact factor: 3.490

2.  Isolation and characterisation of a yeast chromosomal replicator.

Authors:  D T Stinchcomb; K Struhl; R W Davis
Journal:  Nature       Date:  1979-11-01       Impact factor: 49.962

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Authors:  A Toh-e; S Kakimoto
Journal:  Mol Gen Genet       Date:  1975-12-30

4.  Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

Authors:  G K McMaster; G G Carmichael
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

5.  Regulation and characterization of acid and alkaline phosphatase in yeast.

Authors:  A Schurr; E Yagil
Journal:  J Gen Microbiol       Date:  1971-03

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Authors:  A Hinnen; J B Hicks; G R Fink
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

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Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

8.  Translational analysis of the killer-associated virus-like particle dsRNA genome of S. cerevisiae: M dsRNA encodes toxin.

Authors:  K A Bostian; J E Hopper; D T Rogers; D J Tipper
Journal:  Cell       Date:  1980-02       Impact factor: 41.582

9.  Isolation and identification of yeast messenger ribonucleic acids coding for enolase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate kinase.

Authors:  M J Holland; J P Holland
Journal:  Biochemistry       Date:  1978-11-14       Impact factor: 3.162

10.  Regulated high efficiency expression of human interferon-alpha in Saccharomyces cerevisiae.

Authors:  M F Tuite; M J Dobson; N A Roberts; R M King; D C Burke; S M Kingsman; A J Kingsman
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
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  15 in total

Review 1.  Yeast metallothionein and applications in biotechnology.

Authors:  T R Butt; D J Ecker
Journal:  Microbiol Rev       Date:  1987-09

2.  Signal peptide specificity in posttranslational processing of the plant protein phaseolin in Saccharomyces cerevisiae.

Authors:  J H Cramer; K Lea; M D Schaber; R A Kramer
Journal:  Mol Cell Biol       Date:  1987-01       Impact factor: 4.272

3.  Influenza viral (A/WSN/33) hemagglutinin is expressed and glycosylated in the yeast Saccharomyces cerevisiae.

Authors:  M A Jabbar; N Sivasubramanian; D P Nayak
Journal:  Proc Natl Acad Sci U S A       Date:  1985-04       Impact factor: 11.205

4.  Expression of hepatitis B virus core antigen gene in Saccharomyces cerevisiae: synthesis of two polypeptides translated from different initiation codons.

Authors:  A Miyanohara; T Imamura; M Araki; K Sugawara; N Ohtomo; K Matsubara
Journal:  J Virol       Date:  1986-07       Impact factor: 5.103

5.  Molecular cloning and regulated expression of the human c-myc gene in Escherichia coli and Saccharomyces cerevisiae: comparison of the protein products.

Authors:  C Miyamoto; R Chizzonite; R Crowl; K Rupprecht; R Kramer; M Schaber; G Kumar; M Poonian; G Ju
Journal:  Proc Natl Acad Sci U S A       Date:  1985-11       Impact factor: 11.205

Review 6.  Heterologous protein production in yeast.

Authors:  G Gellissen; K Melber; Z A Janowicz; U M Dahlems; U Weydemann; M Piontek; A W Strasser; C P Hollenberg
Journal:  Antonie Van Leeuwenhoek       Date:  1992-08       Impact factor: 2.271

Review 7.  Physiological and technological aspects of large-scale heterologous-protein production with yeasts.

Authors:  M C Hensing; R J Rouwenhorst; J J Heijnen; J P van Dijken; J T Pronk
Journal:  Antonie Van Leeuwenhoek       Date:  1995       Impact factor: 2.271

8.  A novel promoter, derived from the isocitrate lyase gene of Candida tropicalis, inducible with acetate in Saccharomyces cerevisiae.

Authors:  K Umemura; H Atomi; T Kanai; Y Teranishi; M Ueda; A Tanaka
Journal:  Appl Microbiol Biotechnol       Date:  1995-07       Impact factor: 4.813

9.  Regulation of repressible acid phosphatase gene transcription in Saccharomyces cerevisiae.

Authors:  J M Lemire; T Willcocks; H O Halvorson; K A Bostian
Journal:  Mol Cell Biol       Date:  1985-08       Impact factor: 4.272

10.  The impact of phosphate scarcity on pharmaceutical protein production in S. cerevisiae: linking transcriptomic insights to phenotypic responses.

Authors:  Ali Kazemi Seresht; Eva Akke Palmqvist; Lisbeth Olsson
Journal:  Microb Cell Fact       Date:  2011-12-07       Impact factor: 5.328
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