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Literature DB >> 6159584

Synthesis of human fibroblast interferon by E. coli.

D V Goeddel, H M Shepard, E Yelverton, D Leung, R Crea, A Sloma, S Pestka.

Abstract

A cDNA library was constructed using mRNA from human fibroblasts induced with poly(I):poly(C). A bacterial clone containing fibroblast interferon cDNA sequences was identified by hybridization to a cDNA probe synthesized using deoxyoligonucleotide primers which hybridize to fibroblast interferon mRNA specifically. Expression plasmids were constructed which permitted the synthesis in E. coli of 8 x 10(7) units of human fibroblast interferon per liter of culture. The bacterially produced fibroblast interferon is indistinguishable from authentic human fibroblast interferon by several criteria.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1980 PMID： 6159584 PMCID： PMC324219 DOI： 10.1093/nar/8.18.4057

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

27 in total

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8. Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.

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10. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.

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55 in total

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