Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Gene expression: chemical synthesis and molecular cloning of a bacteriophage T5 (T5P25) early promoter.

Literature DB >> 6310520

Gene expression: chemical synthesis and molecular cloning of a bacteriophage T5 (T5P25) early promoter.

J Rommens, D MacKnight, L Pomeroy-Cloney, E Jay.

Abstract

A sixty base pair DNA duplex containing the nucleotide sequence of the bacteriophage T5 early (T5P25) promoter has been constructed using a combination of chemical synthesis and enzymatic methods. Subsequent to cloning into pBR322, the promoter has been demonstrated to be biologically active being capable of directing the efficient expression of genes under its control. This serves as a prototype for an approach to the study of the in vivo structure-function relationships and efficiency of promoters.

Entities: Species

Mesh：

Substances：

Year: 1983 PMID： 6310520 PMCID： PMC326327 DOI： 10.1093/nar/11.17.5921

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

26 in total

1. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.

Authors: W V Shaw
Journal: Methods Enzymol Date: 1975 Impact factor: 1.600

2. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors: T Maniatis; A Jeffrey; H van deSande
Journal: Biochemistry Date: 1975-08-26 Impact factor: 3.162

3. Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures.

Authors: A Dugaiczyk; H W Boyer; H M Goodman
Journal: J Mol Biol Date: 1975-07-25 Impact factor: 5.469

4. Interaction of RNA polymerase with promoters from bacteriophage fd.

Authors: P H Seeburg; C Nüsslein; H Schaller
Journal: Eur J Biochem Date: 1977-03-15

5. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

6. Contacts between Escherichia coli RNA polymerase and a lac operon promoter.

Authors: L Johnsrud
Journal: Proc Natl Acad Sci U S A Date: 1978-11 Impact factor: 11.205

7. Interaction of E. coli RNA polymerase with promotors of coliphage T5: the rates of complex formation and decay and their correlation with in vitro and in vivo transcriptional activity.

Authors: A von Gabain; H Bujard
Journal: Mol Gen Genet Date: 1977-12-09

8. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors: D B Clewell; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1969-04 Impact factor: 11.205

Review 9. Compilation and analysis of Escherichia coli promoter DNA sequences.

Authors: D K Hawley; W R McClure
Journal: Nucleic Acids Res Date: 1983-04-25 Impact factor: 16.971

10. Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd.

Authors: H Schaller; C Gray; K Herrmann
Journal: Proc Natl Acad Sci U S A Date: 1975-02 Impact factor: 11.205

7 in total

1. Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors.

Authors: Ilya A Osterman; Irina V Prokhorova; Vasily O Sysoev; Yulia V Boykova; Olga V Efremenkova; Maxim S Svetlov; Vyacheslav A Kolb; Alexey A Bogdanov; Petr V Sergiev; Olga A Dontsova
Journal: Antimicrob Agents Chemother Date: 2012-01-17 Impact factor: 5.191

Review 2. High-expression of a target gene and high-stability of the plasmid.

Authors: M Kobayashi; Y Kurusu; H Yukawa
Journal: Appl Biochem Biotechnol Date: 1991-02 Impact factor: 2.926

3. Construction of a Co1E1 plasmid bearing inducible high-copy-number phenotype.

Authors: D Bachvarov; E Jay; I Ivanov
Journal: Folia Microbiol (Praha) Date: 1990 Impact factor: 2.099

4. Determination of the optimal aligned spacing between the Shine-Dalgarno sequence and the translation initiation codon of Escherichia coli mRNAs.

Authors: H Chen; M Bjerknes; R Kumar; E Jay
Journal: Nucleic Acids Res Date: 1994-11-25 Impact factor: 16.971

5. RNAII transcribed by IPTG-induced T7 RNA polymerase is non-functional as a replication primer for ColE1-type plasmids in Escherichia coli.

Authors: M Y Chao; M C Kan; S Lin-Chao
Journal: Nucleic Acids Res Date: 1995-05-25 Impact factor: 16.971

6. High-level expression of a chemically synthesized gene for human interferon-gamma using a prokaryotic expression vector.

Authors: E Jay; J Rommens; L Pomeroy-Cloney; D MacKnight; C Lutze-Wallace; P Wishart; D Harrison; W Y Lui; V Asundi; M Dawood
Journal: Proc Natl Acad Sci U S A Date: 1984-04 Impact factor: 11.205

7. Novel Escherichia coli RNA Polymerase Binding Protein Encoded by Bacteriophage T5.

Authors: Evgeny Klimuk; Vladimir Mekler; Darya Lavysh; Marina Serebryakova; Natalia Akulenko; Konstantin Severinov
Journal: Viruses Date: 2020-07-26 Impact factor: 5.048

7 in total