Literature DB >> 6300774

Expression in Escherichia coli of chemically synthesized gene for the human immune interferon.

S Tanaka, T Oshima, K Ohsuye, T Ono, A Mizono, A Ueno, H Nakazato, M Tsujimoto, N Higashi, T Noguchi.   

Abstract

A 454 base pair fragment of double stranded DNA consisting of a gene for a human immune interferon (hIFN-gamma), initiation and termination signals plus appropriate restriction endonuclease sites, was totally synthesized. The synthesis involved preparation of 62 oligodeoxyribonucleotides by rapid, solid phase procedures, and enzymatic ligation of the oligonucleotides. This synthetic gene was expressed in E. coli under the control of the lac UV5 promoter. The product has antiviral activity which was acid labile and completely neutralized by antiserum to hIFN-gamma but not by antiserum to hIFN-alpha or hIFN-beta. Molecular weight of hIFN-gamma produced by E. coli was estimated to be about 32,000 and 17,000 by gel filtration and SDS-polyacrylamide gel electrophoresis respectively.

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Year:  1983        PMID： 6300774      PMCID： PMC325830          DOI： 10.1093/nar/11.6.1707

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  35 in total

1.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

2.  Maximizing gene expression on a plasmid using recombination in vitro.

Authors:  K Backman; M Ptashne
Journal:  Cell       Date:  1978-01       Impact factor: 41.582

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Determinant of cistron specificity in bacterial ribosomes.

Authors:  J Shine; L Dalgarno
Journal:  Nature       Date:  1975-03-06       Impact factor: 49.962

5.  DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

Authors:  E Jay; R Bambara; R Padmanabhan; R Wu
Journal:  Nucleic Acids Res       Date:  1974-03       Impact factor: 16.971

6.  Rapid synthesis of oligodeoxyribonucleotides. III. Effect of added carboxylate ion on the efficiency of internucleotide bond formation. Solid-phase synthesis of the dodecanucleotide, d(pT-A-A-C-T-G-C-T-C-A-C-T).

Authors:  M J Gait; R C Sheppard
Journal:  Nucleic Acids Res       Date:  1979-04       Impact factor: 16.971

7.  Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

Authors:  J Stawinski; T Hozumi; S A Narang; C P Bahl; R Wu
Journal:  Nucleic Acids Res       Date:  1977-02       Impact factor: 16.971

8.  Proteins synthesized in minicells containing plasmid ColE1 and its mutants.

Authors:  J Inselburg; B Applebaum
Journal:  J Bacteriol       Date:  1978-03       Impact factor: 3.490

9.  Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.

Authors:  K Itakura; T Hirose; R Crea; A D Riggs; H L Heyneker; F Bolivar; H W Boyer
Journal:  Science       Date:  1977-12-09       Impact factor: 47.728

10.  Rapid synthesis of oligodeoxyribonucleotides. II. Machine-aided solid-phase syntheses of two nonanucleotides and an octanucleotide.

Authors:  M J Gait; R C Sheppard
Journal:  Nucleic Acids Res       Date:  1977-12       Impact factor: 16.971
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  9 in total

Review 1.  High-expression of a target gene and high-stability of the plasmid.

Authors:  M Kobayashi; Y Kurusu; H Yukawa
Journal:  Appl Biochem Biotechnol       Date:  1991-02       Impact factor: 2.926

2.  Crystallization and preliminary X-ray analysis of a 1:1 complex between a designed monomeric interferon-gamma and its soluble receptor.

Authors:  M Randal; A A Kossiakoff
Journal:  Protein Sci       Date:  1998-04       Impact factor: 6.725

3.  A rapid method for analyzing the ligation products of synthetic oligodeoxyribonucleotides.

Authors:  L U Tan; B R Glick
Journal:  Mol Biol Rep       Date:  1987       Impact factor: 2.316

4.  High-level expression of a chemically synthesized gene for human interferon-gamma using a prokaryotic expression vector.

Authors:  E Jay; J Rommens; L Pomeroy-Cloney; D MacKnight; C Lutze-Wallace; P Wishart; D Harrison; W Y Lui; V Asundi; M Dawood
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

5.  Synthesis of a gene for human growth hormone and its expression in Escherichia coli.

Authors:  M Ikehara; E Ohtsuka; T Tokunaga; Y Taniyama; S Iwai; K Kitano; S Miyamoto; T Ohgi; Y Sakuragawa; K Fujiyama
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

6.  The design of synthetic genes.

Authors:  S R Presnell; S A Benner
Journal:  Nucleic Acids Res       Date:  1988-03-11       Impact factor: 16.971

7.  Reconstitution of the GTP-dependent adenylate cyclase from products of the yeast CYR1 and RAS2 genes in Escherichia coli.

Authors:  I Uno; H Mitsuzawa; K Matsumoto; K Tanaka; T Oshima; T Ishikawa
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

8.  Chemical synthesis of a gene encoding the human complement fragment C5a and its expression in Escherichia coli.

Authors:  W Mandecki; K W Mollison; T J Bolling; B S Powell; G W Carter; J L Fox
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

9.  Isolation of cDNA clones specifying the fourth component of mouse complement and its isotype, sex-limited protein.

Authors:  M Nonaka; M Takahashi; S Natsuume-Sakai; M Nonaka; S Tanaka; A Shimizu; T Honjo
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205
  9 in total

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