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Literature DB >> 6300454

Detection, purification, and characterization of two species of covalently closed circular proviral DNA molecules of bovine leukemia virus.

Abstract

Cocultivation of uninfected and bovine leukemia virus-producing bat cells yielded, in addition to the unintegrated linear DNA duplex, DNA molecules that migrated as 4.4- and 4.8-kilobase-pair DNA fragments in gel electrophoresis. These DNA molecules were purified by acid-phenol extraction and cleaved with restriction endonucleases EcoRI, and HindIII, which have one recognition site each on the bovine leukemia virus proviral DNA. Such cleavage generated DNA molecules of approximately 10.0 and 9.4 kilobase pairs, thus indicating the existence of two species of covalently closed circular molecules of bovine leukemia virus proviral DNA.

Entities: Chemical Species

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Year: 1983 PMID： 6300454 PMCID： PMC256529

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

23 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Covalently closed circular DNA of avian sarcoma virus: purification from nuclei of infected quail tumor cells and measurement by electron microscopy and gel electrophoresis.

Authors: R V Guntaka; O C Richards; P R Shank; H J Kung; N Davidson
Journal: J Mol Biol Date: 1976-09-15 Impact factor: 5.469

3. Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase.

Authors: J M Taylor; R Illmensee; J Summers
Journal: Biochim Biophys Acta Date: 1976-09-06

4. Kinetics of synthesis, structure and purification of avian sarcoma virus-specific DNA made in the cytoplasm of acutely infected cells.

Authors: H E Varmus; S Heasley; H J Kung; H Oppermann; V C Smith; J M Bishop; P R Shank
Journal: J Mol Biol Date: 1978-03-25 Impact factor: 5.469

Detection, purification, and characterization of two species of covalently closed circular proviral DNA molecules of bovine leukemia virus.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. Covalently closed circular DNA of avian sarcoma virus: purification from nuclei of infected quail tumor cells and measurement by electron microscopy and gel electrophoresis.

3. Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase.

4. Kinetics of synthesis, structure and purification of avian sarcoma virus-specific DNA made in the cytoplasm of acutely infected cells.

5. A new method for the purification and identification of covalently closed circular DNA molcules.

6. Selective extraction of polyoma DNA from infected mouse cell cultures.

7. A membrane-filter technique for the detection of complementary DNA.

8. Rapid purification of 70S RNA from media of cells producing RNA tumor viruses.

9. Detection of bovine leukemia virus in B-lymphocytes by the syncytia induction assay.

10. In vitro transmission and propagation of the bovine leukemia virus in monolayer cell cultures.

1. Molecular cloning of covalently closed circular DNA of bovine leukemia virus.

2. The structure of cloned 3'-terminal RNA region of bovine leukemia virus (BLV).