Literature DB >> 198558

Detection of bovine leukemia virus in B-lymphocytes by the syncytia induction assay.

P S Paul, K A Pomeroy, A E Castro, D W Johnson, C C Muscoplat, D K Sorensen.   

Abstract

Bovine peripheral blood lymphocytes (PBL's) from 3 cows and 1 steer infected with bovine leukemia virus (BLV) were separated by fractionation through nylon wool columns into nylon-adherent and nonadherent cell populations. Nylon-adherent cells were enriched in B-lymphocytes, as determined by the presence of surface membrane immunoglobulins (slg), whereas nylon-nonadherent cells or "non-B-lymphocytes" contained few slg-bearing cells. PBL's and separated B- and non-B-lymphocyte populations were assayed for the presence of BLV by the induction of syncytia in bovine embryonic spleen cells. PBL's and B-lymphocyte populations both produced many syncytia, whereas non-B-lymphocytes yielded few or no syncytia. The specificity of syncytia formation by anti-BLV serum. PBL's from 2 control animals were negative for syncytia induction. This study presents further evidence that B-lymphocytes are the target cells for BLV infection.

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Year:  1977        PMID： 198558     DOI： 10.1093/jnci/59.4.1269

Source DB:  PubMed          Journal:  J Natl Cancer Inst        ISSN： 0027-8874            Impact factor:   13.506


  9 in total

1.  The pX region of the bovine leukemia virus is transcribed as a 2.1-kilobase mRNA.

Authors:  R Z Mamoun; T Astier-Gin; R Kettmann; J Deschamps; N Rebeyrotte; B J Guillemain
Journal:  J Virol       Date:  1985-05       Impact factor: 5.103

2.  Phytohemagglutinin activation of the transcription of the bovine leukemia virus genome requires de novo protein synthesis.

Authors:  R Chatterjee; P Gupta; S V Kashmiri; J F Ferrer
Journal:  J Virol       Date:  1985-06       Impact factor: 5.103

3.  Detection, purification, and characterization of two species of covalently closed circular proviral DNA molecules of bovine leukemia virus.

Authors:  S V Kashmiri; R Mehdi; J F Ferrer
Journal:  J Virol       Date:  1983-03       Impact factor: 5.103

4.  Integration of bovine leukemia virus DNA in the bovine genome.

Authors:  R Kettmann; M Meunier-Rotival; J Cortadas; G Cuny; J Ghysdael; M Mammerickx; A Burny; G Bernardi
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

5.  Persistent infection of rabbits with bovine leukemia virus associated with development of immune dysfunction.

Authors:  C R Wyatt; D Wingett; J S White; C D Buck; D Knowles; R Reeves; N S Magnuson
Journal:  J Virol       Date:  1989-11       Impact factor: 5.103

6.  Enumeration of T cells, B cells and monocytes in the peripheral blood of normal and lymphocytotic cattle.

Authors:  P S Paul; D R Senogles; C C Muscoplat; D W Johnson
Journal:  Clin Exp Immunol       Date:  1979-02       Impact factor: 4.330

7.  Bovine lymphocytes: erythrocyte rosettes in normal, lymphomatous and corticosteroid-treated cattle.

Authors:  B N Wilkie; F Caoili; R Jacobs
Journal:  Can J Comp Med       Date:  1979-01

8.  Factors affecting the infectivity of lymphocytes from cattle with bovine leukosis virus.

Authors:  B A Buxton; R D Schultz
Journal:  Can J Comp Med       Date:  1984-10

9.  Inhibition of protein kinase C results in decreased expression of bovine leukemia virus.

Authors:  W A Jensen; B J Wicks-Beard; G L Cockerell
Journal:  J Virol       Date:  1992-07       Impact factor: 5.103
  9 in total

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