Literature DB >> 26042

A new method for the purification and identification of covalently closed circular DNA molcules.

M Zasloff, G D Ginder, G Felsenfeld.   

Abstract

A new technique has been developed for the rapid isolation of covalently closed circular DNA molecules. The procedure is a selective extraction based on differences in the partitioning of covalently closed circular DNA molecules and noncovalently closed species between phenol and water at acid pH and low ionic strength. Under the conditions described, linear as well as nicked circular DNA is extracted into phenol, while covalently closed circular DNA molecules remain in the water phase. The method permits the quantitative isolation of covalently closed circular DNA from either total cellular DNA or partially purified preparations, to a degree of purity comparable with buoyant density procedures.

Entities:  

Mesh：

Substances：

Year:  1978        PMID： 26042      PMCID： PMC342066          DOI： 10.1093/nar/5.4.1139

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  31 in total

1.  Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.

Authors:  M Thomas; R W Davis
Journal:  J Mol Biol       Date:  1975-01-25       Impact factor: 5.469

2.  Discontinuous replication of colicin E1 plasmid deoxyribonucleic acid.

Authors:  J Inselburg; A Oka
Journal:  J Bacteriol       Date:  1975-08       Impact factor: 3.490

3.  Isolation of covalently closed circular DNA of high molecular weight from bacteria.

Authors:  T C Currier; E W Nester
Journal:  Anal Biochem       Date:  1976-12       Impact factor: 3.365

4.  The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneity.

Authors:  M Shure; D E Pulleyblank; J Vinograd
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971

5.  DNA gyrase: an enzyme that introduces superhelical turns into DNA.

Authors:  M Gellert; K Mizuuchi; M H O'Dea; H A Nash
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

6.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

7.  Gene cloning and containment properties of plasmid Col E1 and its derivatives.

Authors:  K A Armstrong; V Hershfield; D R Helinski
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

8.  A simple method for the preparation of large quantities of pure plasmid DNA.

Authors:  G O Humphreys; G A Willshaw; E S Anderson
Journal:  Biochim Biophys Acta       Date:  1975-04-02

9.  Nucleotide sequences of the 5' termini of phi X174 mRNAs synthesized in vitro.

Authors:  L H Smith; K Grohmann; R L Sinsheimer
Journal:  Nucleic Acids Res       Date:  1974-11       Impact factor: 16.971

10.  Mouse mammary tumor virus DNA in infected rat cells: characterization of unintegrated forms.

Authors:  G M Ringold; K R Yamamoto; P R Shank; H E Varmus
Journal:  Cell       Date:  1977-01       Impact factor: 41.582
View more
  78 in total

1.  Chloroplast and nuclear genomes of Chlamydomonas reinhardtii share homology with Escherichia coli genes for DNA replication, repair and transcription.

Authors:  T Oppermann; T H Hong; S J Surzycki
Journal:  Curr Genet       Date:  1989-01       Impact factor: 3.886

2.  Chromosomal assignment of the human 2,3-bisphosphoglycerate mutase gene (BPGM) to region 7q34----7q22.

Authors:  F Barichard; V Joulin; I Henry; M C Garel; C Valentin; R Rosa; M Cohen-Solal; C Junien
Journal:  Hum Genet       Date:  1987-11       Impact factor: 4.132

3.  Molecular characterization of the Escherichia coli K-12 zwf gene encoding glucose 6-phosphate dehydrogenase.

Authors:  D L Rowley; R E Wolf
Journal:  J Bacteriol       Date:  1991-02       Impact factor: 3.490

4.  Characterization of human 5S rRNA genes.

Authors:  P D Sørensen; S Frederiksen
Journal:  Nucleic Acids Res       Date:  1991-08-11       Impact factor: 16.971

5.  Extensive sequence homology in the DNA coding for elongation factor Tu from Escherichia coli and the Chlamydomonas reinhardtii chloroplast.

Authors:  J C Watson; S J Surzycki
Journal:  Proc Natl Acad Sci U S A       Date:  1982-04       Impact factor: 11.205

6.  Hybridization-selected translation of Bombyx mori high-cysteine chorion proteins in Xenopus laevis oocytes.

Authors:  S C Bock; D C Tiemeier; K Mester; M Wu; M R Goldsmith
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

7.  Isolation and characterization of cDNA clones encoding pig gastric mucin.

Authors:  B S Turner; K R Bhaskar; M Hadzopoulou-Cladaras; R D Specian; J T LaMont
Journal:  Biochem J       Date:  1995-05-15       Impact factor: 3.857

8.  DNA-mediated alteration of the reversion frequency of transformed NIH/3T3 cells.

Authors:  R F Baker
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

9.  Induction of tumors and generation of recovered sarcoma viruses by, and mapping of deletions in, two molecularly cloned src deletion mutants.

Authors:  L H Wang; B Edelstein; B J Mayer
Journal:  J Virol       Date:  1984-06       Impact factor: 5.103

10.  Sequence organization within and flanking clusters of 5S ribosomal RNA genes in Tetrahymena.

Authors:  D S Pederson; M C Yao; A R Kimmel; M A Gorovsky
Journal:  Nucleic Acids Res       Date:  1984-03-26       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.