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Literature DB >> 6296773

Transcription initiation site of rat ribosomal DNA.

Abstract

The sequence of 1,100 nucleotides surrounding the transcription initiation site of a cloned rat ribosomal RNA gene (rDNA) has been determined. The location of the 5' terminus of 45S pre-rRNA was determined by S1 nuclease mapping, reverse transcriptase elongation and confirmed by in vitro capping of 45S rRNA and in vitro transcription. Two different plasmid subclones, from two separate genomic clones of rat rDNA, contained the identical sequence surrounding the transcription initiation site: -10GGAGATATAT 1GCTGACACGC TGTCCTTTTG+20. Relatively long, greater than 15 base pairs, regions of sequence homology were found when the sequences of the initiation regions of rat and mouse rDNA (Urano, Y., Kominami, R., Mishima, Y., and Muramatsu, M., Nucleic Acids Res. 8, 6043-6058, 1980) were compared. When both the rat and mouse sequences were compared to that of human rDNA (G. Wilson, personal communication) a sequence of 15 nucleotides immediately following the initiation sites were found to be preserved.

Entities: Gene Species

Mesh：

Substances：

Year: 1982 PMID： 6296773 PMCID： PMC327008 DOI： 10.1093/nar/10.22.7345

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

32 in total

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Journal: Cell Date: 1977-11 Impact factor: 41.582

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Journal: Proc Natl Acad Sci U S A Date: 1977-10 Impact factor: 11.205

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Journal: J Biol Chem Date: 1970-04-25 Impact factor: 5.157

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Journal: J Biol Chem Date: 1975-07-10 Impact factor: 5.157

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Journal: J Biol Chem Date: 1975-08-10 Impact factor: 5.157

42 in total

1. Domains of the rat rDNA promoter must be aligned stereospecifically.

Authors: W Q Xie; L I Rothblum
Journal: Mol Cell Biol Date: 1992-03 Impact factor: 4.272

2. Analysis of the rat ribosomal DNA promoter: characterization of linker-scanning mutants and of the binding of UBF.

Authors: W Xie; D J O'Mahony; S D Smith; D Lowe; L I Rothblum
Journal: Nucleic Acids Res Date: 1992-04-11 Impact factor: 16.971

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Authors: S T Jacob; J Zhang; L C Garg; C B Book
Journal: Mol Cell Biochem Date: 1991 May 29-Jun 12 Impact factor: 3.396

4. E1BF is an essential RNA polymerase I transcription factor with an intrinsic protein kinase activity that can modulate rRNA gene transcription.

Authors: J Zhang; H F Niu; S T Jacob
Journal: Proc Natl Acad Sci U S A Date: 1991-10-01 Impact factor: 11.205

5. Purification and characterization of a novel factor which stimulates rat ribosomal gene transcription in vitro by interacting with enhancer and core promoter elements.

Authors: J Zhang; S T Jacob
Journal: Mol Cell Biol Date: 1990-10 Impact factor: 4.272

6. Effects of single-base substitutions within the Acanthamoeba castellanii rRNA promoter on transcription and on binding of transcription initiation factor and RNA polymerase I.

Authors: P Kownin; E Bateman; M R Paule
Journal: Mol Cell Biol Date: 1988-02 Impact factor: 4.272

7. Transfection of mouse ribosomal DNA into rat cells: faithful transcription and processing.

Authors: V B Vance; E A Thompson; L H Bowman
Journal: Nucleic Acids Res Date: 1985-10-25 Impact factor: 16.971

8. Locations and contexts of sequences that hybridize to poly(dG-dT).(dC-dA) in mammalian ribosomal DNAs and two X-linked genes.

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Journal: Nucleic Acids Res Date: 1988-02-11 Impact factor: 16.971

9. Activation of RNA polymerase III transcription of human Alu repetitive elements by adenovirus type 5: requirement for the E1b 58-kilodalton protein and the products of E4 open reading frames 3 and 6.

Authors: B Panning; J R Smiley
Journal: Mol Cell Biol Date: 1993-06 Impact factor: 4.272

10. Characterization of factors that direct transcription of rat ribosomal DNA.

Authors: S D Smith; E Oriahi; D Lowe; H F Yang-Yen; D O'Mahony; K Rose; K Chen; L I Rothblum
Journal: Mol Cell Biol Date: 1990-06 Impact factor: 4.272