Literature DB >> 19730

Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.

J Casey, N Davidson.   

Abstract

The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DNA:DNA rates are negligible and RNA:DNA rates close to optimal.

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Year:  1977        PMID： 19730      PMCID： PMC343772          DOI： 10.1093/nar/4.5.1539

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  21 in total

1.  Buoyant density sedimentation of macromolecules in sodium iothalamate density gradients.

Authors:  P Serwer
Journal:  J Mol Biol       Date:  1975-03-05       Impact factor: 5.469

2.  Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.

Authors:  J M Bailey; N Davidson
Journal:  Anal Biochem       Date:  1976-01       Impact factor: 3.365

3.  Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.

Authors:  J MARMUR; P DOTY
Journal:  J Mol Biol       Date:  1962-07       Impact factor: 5.469

4.  Renaturation of bacteriophage phiX174 DNA-RNA hybrid: RNA length effect and nucleation rate constant.

Authors:  J R Hutton; J G Wetmur
Journal:  J Mol Biol       Date:  1973-07-15       Impact factor: 5.469

5.  Use of formamide in nucleic acid reassociation.

Authors:  B J Schmeckpeper; K D Smith
Journal:  Biochemistry       Date:  1972-03-28       Impact factor: 3.162

6.  Hybridization of mitochondrial transfer RNA's with mitochondrial and nuclear DNA of grande (wild type) yeast.

Authors:  J Casey; M Cohen; M Rabinowitz; H Fukuhara; G S Getz
Journal:  J Mol Biol       Date:  1972-02-14       Impact factor: 5.469

7.  Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. VII. Mapping the ribosomal RNA genes of plasmid F14.

Authors:  R C Deonier; E Otsubo; H J Lee; N Davidson
Journal:  J Mol Biol       Date:  1974-11-15       Impact factor: 5.469

8.  In vitro iodination of DNA. Maximizing iodination while minimizing degradation; use of buoyant density shifts for DNA-DNA hybrid isolation.

Authors:  J M Orosz; J G Wetmur
Journal:  Biochemistry       Date:  1974-12-31       Impact factor: 3.162

9.  Analysis of repeating DNA sequences by reassociation.

Authors:  R J Britten; D E Graham; B R Neufeld
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

10.  Kinetic complexity of RNA molecules.

Authors:  M L Birnstiel; B H Sells; I F Purdom
Journal:  J Mol Biol       Date:  1972-01-14       Impact factor: 5.469
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  222 in total

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Authors:  S Chandwani; A Kaul
Journal:  Indian J Pediatr       Date:  1995 Jan-Feb       Impact factor: 1.967

2.  Counting and size classification of active soil bacteria by fluorescence in situ hybridization with an rRNA oligonucleotide probe.

Authors:  H Christensen; M Hansen; J Sorensen
Journal:  Appl Environ Microbiol       Date:  1999-04       Impact factor: 4.792

3.  Control of adenovirus early gene expression: posttranscriptional control mediated by both viral and cellular gene products.

Authors:  M G Katze; H Persson; L Philipson
Journal:  Mol Cell Biol       Date:  1981-09       Impact factor: 4.272

Review 4.  Molecular biology made easy. The polymerase chain reaction.

Authors:  A M Clarke; N P Mapstone; P Quirke
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5.  Mutational alterations within the simian virus 40 leader segment generate altered 16S and 19S mRNA's.

Authors:  L P Villarreal; R T White; P Berg
Journal:  J Virol       Date:  1979-01       Impact factor: 5.103

6.  Mapping early transcripts of herpes simplex virus type 1 by electron microscopy.

Authors:  J R Stringer; L E Holland; E K Wagner
Journal:  J Virol       Date:  1978-07       Impact factor: 5.103

7.  Characterization of the env gene in avian oncoviruses by heteroduplex mapping.

Authors:  S F Hu; M M Lai; P K Vogt
Journal:  J Virol       Date:  1978-09       Impact factor: 5.103

8.  Polyoma virus giant RNAs contain tandem repeats of the nucleotide sequence of the entire viral genome.

Authors:  N H Acheson
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

9.  Extensive sequence homology in the DNA coding for elongation factor Tu from Escherichia coli and the Chlamydomonas reinhardtii chloroplast.

Authors:  J C Watson; S J Surzycki
Journal:  Proc Natl Acad Sci U S A       Date:  1982-04       Impact factor: 11.205

10.  Cloned Drosophila alcohol dehydrogenase genes are correctly expressed after transfection into Drosophila cells in culture.

Authors:  C Benyajati; J F Dray
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205
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