| Literature DB >> 1033038 |
Abstract
Mapping with the restriction enzymes Sal 1 and R1 has generated a picture of the organization of Dictyostelium ribosomal DNA. The DNA which codes for 17S and 25S ribosomal RNAs is located within a stretch of repetitive DNA at least 38,000 base pairs long. This repeated unit includes 5S DNA, linked to 25S DNA. Two techniques were especially useful in the mapping: "cloning" 14S + 25S DNA on the plasmid pMB9 to amplify individual R1 fragments, and digesting DNA with R1 in the presence of the antibiotic distamycin A to produce specific partial digestion products.Entities:
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Year: 1976 PMID: 1033038 DOI: 10.1016/0092-8674(76)90088-x
Source DB: PubMed Journal: Cell ISSN: 0092-8674 Impact factor: 41.582