Literature DB >> 6248731

Construction and properties of a new cloning vehicle, allowing direct screening for recombinant plasmids.

U Rüther.   

Abstract

pUR2, a certified B2(EK2) vector, allows easy isolation of variants containing cloned EcoRI-fragments. Bacteria harboring plasmids without inserts make blue colonies on indicator-plates, whilst those harboring recombinant plasmids make white colonies.

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Mesh：

Year:  1980        PMID： 6248731     DOI： 10.1007/bf00270503

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  10 in total

1.  Amino acid sequence of beta-galactosidase. IV. Sequence of an alpha-complementing cyanogen bromide peptide, residues 3 to 92.

Authors:  K E Langley; A V Fowler; I Zabin
Journal:  J Biol Chem       Date:  1975-04-10       Impact factor: 5.157

2.  Molecular basis of beta-galactosidase alpha-complementation.

Authors:  K E Langley; M R Villarejo; A V Fowler; P J Zamenhof; I Zabin
Journal:  Proc Natl Acad Sci U S A       Date:  1975-04       Impact factor: 11.205

3.  Replica plating and indirect selection of bacterial mutants.

Authors:  J LEDERBERG; E M LEDERBERG
Journal:  J Bacteriol       Date:  1952-03       Impact factor: 3.490

4.  pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

Authors:  J G Sutcliffe
Journal:  Nucleic Acids Res       Date:  1978-08       Impact factor: 16.971

5.  Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

Authors:  J Messing; B Gronenborn; B Müller-Hill; P Hans Hopschneider
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

6.  Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites.

Authors:  B Gronenborn; J Messing
Journal:  Nature       Date:  1978-03-23       Impact factor: 49.962

7.  Genetic mapping of the regulator and operator genes of the lac operon.

Authors:  J Davies; F Jacob
Journal:  J Mol Biol       Date:  1968-09-28       Impact factor: 5.469

8.  Calcium-dependent bacteriophage DNA infection.

Authors:  M Mandel; A Higa
Journal:  J Mol Biol       Date:  1970-10-14       Impact factor: 5.469

9.  Characterization by in vitro complementation of a peptide corresponding to an operator-proximal segment of the beta-galactosidase structural gene of Escherichia coli.

Authors:  A Ullmann; F Jacob; J Monod
Journal:  J Mol Biol       Date:  1967-03-14       Impact factor: 5.469

10.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688
  10 in total
  48 in total

1.  A rewired green fluorescent protein: folding and function in a nonsequential, noncircular GFP permutant.

Authors:  Philippa J Reeder; Yao-Ming Huang; Jonathan S Dordick; Christopher Bystroff
Journal:  Biochemistry       Date:  2010-12-03       Impact factor: 3.162

2.  Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.

Authors:  K C Murphy
Journal:  J Bacteriol       Date:  1998-04       Impact factor: 3.490

3.  A functional analysis of T-DNA gene 6b: the fine tuning of cytokinin effects on shoot development.

Authors:  K Spanier; J Schell; P H Schreier
Journal:  Mol Gen Genet       Date:  1989-10

4.  Development of a reporter peptide that catalytically produces a fluorescent signal through α-complementation.

Authors:  Kotaro Nishiyama; Norikazu Ichihashi; Yasuaki Kazuta; Tetsuya Yomo
Journal:  Protein Sci       Date:  2015-04-02       Impact factor: 6.725

5.  Probing the sequence-specific interaction of the cyclic AMP receptor protein with DNA by site-directed mutagenesis.

Authors:  M E Gent; A M Gronenborn; R W Davies; G M Clore
Journal:  Biochem J       Date:  1987-03-15       Impact factor: 3.857

6.  Mutational analysis of the Bradyrhizobium japonicum common nod genes and further nod box-linked genomic DNA regions.

Authors:  M Göttfert; J W Lamb; R Gasser; J Semenza; H Hennecke
Journal:  Mol Gen Genet       Date:  1989-02

7.  Location and nucleotide sequence of two tRNA genes and a tRNA pseudo-gene in the maize mitochondrial genome: evidence for the transcription of a chloroplast gene in mitochondria.

Authors:  H Wintz; J M Grienenberger; J H Weil; D M Lonsdale
Journal:  Curr Genet       Date:  1988-03       Impact factor: 3.886

8.  korA function of promiscuous plasmid RK2: an autorepressor that inhibits expression of host-lethal gene kilA and replication gene trfA.

Authors:  C Young; A S Prince; D H Figurski
Journal:  Proc Natl Acad Sci U S A       Date:  1985-11       Impact factor: 11.205

9.  The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a non-reverting his4 deletion mutant, and development of HIS4-based replicating and integrating plasmids.

Authors:  D I Crane; S J Gould
Journal:  Curr Genet       Date:  1994 Nov-Dec       Impact factor: 3.886

10.  Expression of the fixR-nifA operon in Bradyrhizobium japonicum depends on a new response regulator, RegR.

Authors:  E Bauer; T Kaspar; H M Fischer; H Hennecke
Journal:  J Bacteriol       Date:  1998-08       Impact factor: 3.490
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