Literature DB >> 6324114

Analysis of a eukaryotic beta-galactosidase gene: the N-terminal end of the yeast Kluyveromyces lactis protein shows homology to the Escherichia coli lacZ gene product.

K D Breunig, U Dahlems, S Das, C P Hollenberg.   

Abstract

The LAC4 gene of Kluyveromyces lactis, encoding the enzyme beta-galactosidase was mapped on a cloned DNA fragment and the sequence of the 5' end was determined. This sequence includes the 5' regulatory region involved in the induction by lactose and the N-terminal end of the protein coding region. Comparison of the deduced amino acid sequence of this eukaryotic enzyme with the N-terminal end of the Escherichia coli beta-galactosidase revealed substantial homology. Two major RNA initiation sites were mapped at -115 and -105. A number of structural peculiarities of the 5'non-coding region are discussed as in comparison to Saccharomyces cerevisiae genes.

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Year:  1984        PMID： 6324114      PMCID： PMC318665          DOI： 10.1093/nar/12.5.2327

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  24 in total

1.  Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae.

Authors:  M Smith; D W Leung; S Gillam; C R Astell; D L Montgomery; B D Hall
Journal:  Cell       Date:  1979-04       Impact factor: 41.582

2.  Heterogeneity and 5'-terminal structures of the late RNAs of simian virus 40.

Authors:  P K Ghosh; V B Reddy; J Swinscoe; P Lebowitz; S M Weissman
Journal:  J Mol Biol       Date:  1978-12-25       Impact factor: 5.469

3.  Organisation and sequences at the 5' end of a cloned complete ovalbumin gene.

Authors:  F Gannon; K O'Hare; F Perrin; J P LePennec; C Benoist; M Cochet; R Breathnach; A Royal; A Garapin; B Cami; P Chambon
Journal:  Nature       Date:  1979-03-29       Impact factor: 49.962

4.  Estimation of secondary structure in ribonucleic acids.

Authors:  I Tinoco; O C Uhlenbeck; M D Levine
Journal:  Nature       Date:  1971-04-09       Impact factor: 49.962

5.  A simple method for DNA restriction site mapping.

Authors:  H O Smith; M L Birnstiel
Journal:  Nucleic Acids Res       Date:  1976-09       Impact factor: 16.971

6.  The ovalbumin gene-sequence of putative control regions.

Authors:  C Benoist; K O'Hare; R Breathnach; P Chambon
Journal:  Nucleic Acids Res       Date:  1980-01-11       Impact factor: 16.971

7.  Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

Authors:  G K McMaster; G G Carmichael
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

8.  Molecular cloning and expression in E. coli of a yeast gene coding for beta-galactosidase.

Authors:  R C Dickson; J S Markin
Journal:  Cell       Date:  1978-09       Impact factor: 41.582

9.  Transformation of yeast.

Authors:  A Hinnen; J B Hicks; G R Fink
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  15 in total

1.  Functional relationship among TATA sequences, gene induction and transcription initiation in the beta-galactosidase, LAC4, gene from Kluyveromyces lactis.

Authors:  A G Ficca; C P Hollenberg
Journal:  Curr Genet       Date:  1989-04       Impact factor: 3.886

2.  Structure of a beta-galactosidase gene of Bacillus stearothermophilus.

Authors:  H Hirata; T Fukazawa; S Negoro; H Okada
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

3.  Glucose repression of LAC gene expression in yeast is mediated by the transcriptional activator LAC9.

Authors:  K D Breunig
Journal:  Mol Gen Genet       Date:  1989-04

4.  Properties of promoters cloned randomly from the Saccharomyces cerevisiae genome.

Authors:  G M Santangelo; J Tornow; C S McLaughlin; K Moldave
Journal:  Mol Cell Biol       Date:  1988-10       Impact factor: 4.272

5.  Coregulation of the Kluyveromyces lactis lactose permease and beta-galactosidase genes is achieved by interaction of multiple LAC9 binding sites in a 2.6 kbp divergent promoter.

Authors:  A Gödecke; W Zachariae; A Arvanitidis; K D Breunig
Journal:  Nucleic Acids Res       Date:  1991-10-11       Impact factor: 16.971

6.  Expression of an endogenous and a heterologous gene in Candida maltosa by using a promoter of a newly-isolated phosphoglycerate kinase (PGK) gene.

Authors:  Y Masuda; S M Park; M Ohkuma; A Ohta; M Takagi
Journal:  Curr Genet       Date:  1994-05       Impact factor: 3.886

7.  A transcriptional barrier to expression of cloned toxin genes of the linear plasmid k1 of Kluyveromyces lactis: evidence that native k1 has novel promoters.

Authors:  M A Romanos; A Boyd
Journal:  Nucleic Acids Res       Date:  1988-08-11       Impact factor: 16.971

8.  Expression and nucleotide sequence of the Lactobacillus bulgaricus beta-galactosidase gene cloned in Escherichia coli.

Authors:  B F Schmidt; R M Adams; C Requadt; S Power; S E Mainzer
Journal:  J Bacteriol       Date:  1989-02       Impact factor: 3.490

9.  Functional homology between the yeast regulatory proteins GAL4 and LAC9: LAC9-mediated transcriptional activation in Kluyveromyces lactis involves protein binding to a regulatory sequence homologous to the GAL4 protein-binding site.

Authors:  K D Breunig; P Kuger
Journal:  Mol Cell Biol       Date:  1987-12       Impact factor: 4.272

10.  Nucleotide sequence and transcription analysis of a linear DNA plasmid associated with the killer character of the yeast Kluyveromyces lactis.

Authors:  M J Stark; A J Mileham; M A Romanos; A Boyd
Journal:  Nucleic Acids Res       Date:  1984-08-10       Impact factor: 16.971
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