Literature DB >> 6288765

Detection of immunoglobulin M antibody to Epstein-Barr virus by use of an enzyme-labeled antigen.

H Schmitz.   

Abstract

Immunoglobulin M (IgM) antibodies to Epstein-Barr virus were detected by using microtiter plates coated with anti-mu-chain antiserum and enzyme-labeled Epstein-Barr virus antigen. Optimum conditions for labeling were determined. The addition of unlabeled control antigen to the enzyme-labeled antigen was effective in reducing background reactions. Rheumatoid factor no longer interfered. Blocking of specific IgM antibody by IgG also was no longer observed. Four different methods for the detection of acute Epstein-Barr virus infections were compared. A combination of the enzyme-labeled antigen-IgM test with the detection of antibodies to either Epstein-Barr nuclear antigen or heterophile antigen was highly sensitive and specific. By changing the solid phase, IgA antibodies to Epstein-Barr virus could be detected.

Entities:  

Mesh：

Substances：

Year:  1982        PMID： 6288765      PMCID： PMC272361          DOI： 10.1128/jcm.16.2.361-366.1982

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Detection of antibodies to Sendai virus by enzyme-linked immunosorbent assay (ELISA).

Authors:  H C Ertl; W Gerlich; U H Koszinowski
Journal:  J Immunol Methods       Date:  1979       Impact factor: 2.303

2.  Rheumatoid factor as a cause of positive reactions in tests for Epstein-Barr virus-specific IgM antibodies.

Authors:  G Henle; E T Lennette; M A Alspaugh; W Henle
Journal:  Clin Exp Immunol       Date:  1979-06       Impact factor: 4.330

3.  Improved detection of virus-specific IgM antibodies. Elimination of non-specific IgM binding.

Authors:  H Schmitz
Journal:  J Gen Virol       Date:  1978-08       Impact factor: 3.891

4.  Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus.

Authors:  H Schmitz; H W Doerr; D Kampa; A Vogt
Journal:  J Clin Microbiol       Date:  1977-06       Impact factor: 5.948

5.  Heterophil antigen in bovine sera detectable by immune adherence hemagglutination with infectious mononucleosis sera.

Authors:  E T Lennette; G Henle; W Henle; C A Horwitz
Journal:  Infect Immun       Date:  1978-03       Impact factor: 3.441

6.  Latex test for serodiagnosis of infectious mononucleosis.

Authors:  B A Levey; T M Lo; K E Caldwell; M A Fletcher
Journal:  J Clin Microbiol       Date:  1980-03       Impact factor: 5.948

7.  Persisting oncogenic herpesvirus induced by the tumour promotor TPA.

Authors:  H zur Hausen; F J O'Neill; U K Freese; E Hecker
Journal:  Nature       Date:  1978-03-23       Impact factor: 49.962

8.  Antibodies to Epstein-Barr virus-associated nuclear antigen in infectious mononucleosis.

Authors:  G Henie; W Henle; C A Horwitz
Journal:  J Infect Dis       Date:  1974-09       Impact factor: 5.226

9.  Detection of IgM antibodies to cytomegalovirus (CMV) using an enzyme-labelled antigen (ELA).

Authors:  H Schmitz; U von Deimling; B Flehmig
Journal:  J Gen Virol       Date:  1980-09       Impact factor: 3.891

10.  IgM antibodies specific for Epstein-Barr virus in infectious mononucleosis without heterophil antibodies.

Authors:  J Nikoskelainen; J Leikola; E Klemola
Journal:  Br Med J       Date:  1974-10-12
View more
  11 in total

1.  Indirect alkaline phosphatase immunoenzymatic staining for the detection of antibodies to Epstein-Barr virus-induced virus capsid antigens and early antigens.

Authors:  M Musiani; M Zerbini; G Gentilomi; M La Placa
Journal:  J Clin Microbiol       Date:  1986-02       Impact factor: 5.948

2.  An immunoperoxidase assay for the detection of specific IgA antibody in Epstein-Barr virus infections.

Authors:  R Cevenini; M Donati; F Rumpianesi; A Moroni; P Paolucci
Journal:  J Clin Pathol       Date:  1984-04       Impact factor: 3.411

3.  Rapid diagnosis of acute Epstein-Barr virus infection by an indirect enzyme-linked immunosorbent assay for specific immunoglobulin M (IgM) antibody without rheumatoid factor and specific IgG interference.

Authors:  D W Ho; P R Field; A L Cunningham
Journal:  J Clin Microbiol       Date:  1989-05       Impact factor: 5.948

4.  Detection of specific immunoglobulin M antibody to rubella virus by use of an enzyme-labeled antigen.

Authors:  C Bonfanti; O Meurman; P Halonen
Journal:  J Clin Microbiol       Date:  1985-06       Impact factor: 5.948

5.  Assay of measles virus IgM and IgG class antibodies by use of peroxidase-labelled viral antigens.

Authors:  J Salonen; R Vainionpää; P Halonen
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574

6.  Development and evaluation of a capture enzyme-linked immunosorbent assay for determination of rubella immunoglobulin M using monoclonal antibodies.

Authors:  I Gerna; M Zannino; M G Revello; E Petruzzelli; M Dovis
Journal:  J Clin Microbiol       Date:  1987-06       Impact factor: 5.948

7.  Detection of specific immunoglobulin M antibody to different flaviviruses by use of enzyme-labeled antigens.

Authors:  H Schmitz; P Emmerich
Journal:  J Clin Microbiol       Date:  1984-05       Impact factor: 5.948

8.  Antibody class capture assays for varicella-zoster virus.

Authors:  B Forghani; C K Myoraku; K W Dupuis; N J Schmidt
Journal:  J Clin Microbiol       Date:  1984-05       Impact factor: 5.948

9.  Detection of antichlamydial immunoglobulin G and M antibodies by enzyme-linked immunosorbent assay.

Authors:  J B Mahony; J Schachter; M A Chernesky
Journal:  J Clin Microbiol       Date:  1983-08       Impact factor: 5.948

Review 10.  Detection of viral antigens, particles, and early antibodies in diagnosis.

Authors:  M A Chernesky; J B Mahony
Journal:  Yale J Biol Med       Date:  1984 Sep-Oct
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.