Literature DB >> 6255087

Detection of IgM antibodies to cytomegalovirus (CMV) using an enzyme-labelled antigen (ELA).

H Schmitz, U von Deimling, B Flehmig.   

Abstract

We have applied a peroxidase enzyme-labelled antigen (ELA) for the detection of IgM antibodies to cytomegalovirus: microtitre plates were coated with anti-IgM immunoglobulin. The IgM fraction of human serum was selectively bound to the precoated plates and the virus-specific IgM antibody was then detected by the enzyme-labelled antigen. A very efficient technique for the labelling of virus antigen is described. The IgM antibody was detected simply and specifically, Rheumatoid factor IgM did not interfere with this test.

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Year:  1980        PMID： 6255087     DOI： 10.1099/0022-1317-50-1-59

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  32 in total

1.  Antibody-capture enzyme-linked immunosorbent assays that use enzyme-labelled antigen for detection of virus-specific immunoglobulin M, A and G in patients with varicella or herpes zoster.

Authors:  A M van Loon; J T van der Logt; F W Heessen; M C Heeren; J Zoll
Journal:  Epidemiol Infect       Date:  1992-02       Impact factor: 2.451

2.  Specific serum immunoglobulin D, detected by antibody capture enzyme-linked immunosorbent assay (ELISA), in cytomegalovirus infection.

Authors:  J Mortensen; S L Nielsen; I Sørensen; H K Andersen
Journal:  Clin Exp Immunol       Date:  1989-02       Impact factor: 4.330

3.  Detection of specific immunoglobulin M antibody to rubella virus by use of an enzyme-labeled antigen.

Authors:  C Bonfanti; O Meurman; P Halonen
Journal:  J Clin Microbiol       Date:  1985-06       Impact factor: 5.948

4.  Immunoglobulin M antibody capture enzyme-linked immunosorbent assay for diagnosis of St. Louis encephalitis.

Authors:  T P Monath; R R Nystrom; R E Bailey; C H Calisher; D J Muth
Journal:  J Clin Microbiol       Date:  1984-10       Impact factor: 5.948

5.  Assay of measles virus IgM and IgG class antibodies by use of peroxidase-labelled viral antigens.

Authors:  J Salonen; R Vainionpää; P Halonen
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574

6.  Antibodies to cytomegalovirus-induced pre-early nuclear antigen in the anticomplement-immunofluorescent test in comparison to IgG and IgM antibodies in the indirect and direct enzyme-linked immunosorbent assay in diagnosing cytomegalovirus infections.

Authors:  L Gärtner; I Orstavik
Journal:  Arch Virol       Date:  1984       Impact factor: 2.574

7.  The production of monoclonal antibodies to rubella haemagglutinin and their use in antibody-capture assays for rubella-specific IgM.

Authors:  R S Tedder; J L Yao; M J Anderson
Journal:  J Hyg (Lond)       Date:  1982-04

8.  Hepatitis B e-antigen titres for evaluating infectivity during hepatitis B virus infection.

Authors:  E G Lindenschmidt; C F Granato; C Salefsky; R Laufs; H Henning
Journal:  Klin Wochenschr       Date:  1984-03-01

9.  Immunoglobulin M antibodies to hepatitis B core antigen: evaluation of enzyme immunoassay for diagnosis of hepatitis B virus infection.

Authors:  M Roggendorf; F Deinhardt; G G Frösner; R Scheid; B Bayerl; R Zachoval
Journal:  J Clin Microbiol       Date:  1981-04       Impact factor: 5.948

10.  Development and evaluation of a capture enzyme-linked immunosorbent assay for determination of rubella immunoglobulin M using monoclonal antibodies.

Authors:  I Gerna; M Zannino; M G Revello; E Petruzzelli; M Dovis
Journal:  J Clin Microbiol       Date:  1987-06       Impact factor: 5.948
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