| Literature DB >> 195977 |
H Schmitz, H W Doerr, D Kampa, A Vogt.
Abstract
A sensitive, solid-phase enzyme immunoassay for the detection of immunoglobulin M antibodies to cytomegalovirus is described. The results of the enzyme immunoassay correlated well with those obtained by an indirect immunofluorescence method. Horseradish peroxidase proved to be a more sensitive label than alkaline phosphatase. Nonspecific reactions, occurring with commercially available cytomegalovirus antigens, could be avoided by using a nuclear antigen prepared from sonically disrupted nuclei of cytomegalovirus-infected cells.Entities:
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Year: 1977 PMID: 195977 PMCID: PMC274669 DOI: 10.1128/jcm.5.6.629-634.1977
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948