Literature DB >> 2422201

Indirect alkaline phosphatase immunoenzymatic staining for the detection of antibodies to Epstein-Barr virus-induced virus capsid antigens and early antigens.

M Musiani, M Zerbini, G Gentilomi, M La Placa.   

Abstract

An indirect alkaline phosphatase immunoenzymatic staining technique was developed for the detection of antibodies against Epstein-Barr virus-induced virus capsid antigens and early antigens in cell smears. The presence of antibodies against Epstein-Barr virus-induced virus capsid antigens and early antigens was revealed by a dark blue staining of cells expressing the antigens. The alkaline phosphatase assay gave a permanent record of the reaction that could be visualized under an ordinary light microscope. The titers obtained with this assay on 91 serum samples were significantly correlated with the titers obtained with an immunofluorescence technique.

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Year:  1986        PMID： 2422201      PMCID： PMC268631          DOI： 10.1128/jcm.23.2.302-304.1986

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Enzyme immunoassay for detection of antibody to Epstein-Barr virus-specific early antigen.

Authors:  D J Garnlund; P H Levine; D A Fuccillo
Journal:  J Clin Microbiol       Date:  1979-11       Impact factor: 5.948

2.  Epstein-Barr virus antibodies in the cerebrospinal fluid. A case of infectious mononucleosis with encephalitis.

Authors:  J H Joncas; L Chicoine; F Thivierge; M Bertrand
Journal:  Am J Dis Child       Date:  1974-02

3.  Epstein-Barr virus-specific serology in immunologically compromised individuals.

Authors:  W Henle; G Henle
Journal:  Cancer Res       Date:  1981-11       Impact factor: 12.701

4.  Detection of antibodies to Epstein-Barr virus capsid antigen by immune adherence hemagglutination.

Authors:  E T Lennette; E Ward; G Henle; W Henle
Journal:  J Clin Microbiol       Date:  1982-01       Impact factor: 5.948

5.  Rapid detection of antibodies against cytomegalovirus induced immediate early and early antigens by an enzyme linked immunosorbent assay.

Authors:  M Musiani; C Carpi; M Zerbini
Journal:  J Clin Pathol       Date:  1984-02       Impact factor: 3.411

6.  Can Epstein-Barr virus infect and transform all the B-lymphocytes of human cord blood?

Authors:  M Zerbini; I Ernberg
Journal:  J Gen Virol       Date:  1983-03       Impact factor: 3.891

7.  Rapid detection of human cytomegalovirus and herpesvirus hominis IgM antibody by the immunoperoxidase technique.

Authors:  G Gerna; R W Chambers
Journal:  Intervirology       Date:  1977       Impact factor: 1.763

8.  Detection of immunoglobulin M antibody to Epstein-Barr virus by use of an enzyme-labeled antigen.

Authors:  H Schmitz
Journal:  J Clin Microbiol       Date:  1982-08       Impact factor: 5.948

9.  Enumeration in whole peripheral blood of lymphocytes bearing receptors for Fc(gamma) and C3b using alkaline phosphatase-labelled reagents.

Authors:  E O Pepys; M B Pepys
Journal:  J Immunol Methods       Date:  1980       Impact factor: 2.303

10.  Detection of active Epstein-Barr infection in pregnant women.

Authors:  S Costa; R Barrasso; P Terzano; M Zerbini; C Carpi; M Musiani
Journal:  Eur J Clin Microbiol       Date:  1985-06       Impact factor: 3.267
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  2 in total

1.  Comparison of the immune response to Epstein-Barr virus and cytomegalovirus in sera and synovial fluids of patients with rheumatoid arthritis.

Authors:  M Musiani; M Zerbini; S Ferri; M Plazzi; G Gentilomi; M La Placa
Journal:  Ann Rheum Dis       Date:  1987-11       Impact factor: 19.103

2.  Immunocytochemical detection of antibodies to Epstein-Barr virus nuclear antigen by a streptavidin-biotin-complex assay.

Authors:  M Musiani; M Zerbini; M Plazzi; G Gentilomi; M La Placa
Journal:  J Clin Microbiol       Date:  1988-05       Impact factor: 5.948
  2 in total

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