Literature DB >> 6287023

mRNA- and DNA-directed synthesis of herpes simplex virus-coded exonuclease in Xenopus laevis oocytes.

C M Preston, M G Cordingley.   

Abstract

Microinjection of herpes simplex virus (HSV)-infected cell mRNA into Xenopus laevis oocytes resulted in the production of a new exonuclease activity. This enzyme strongly resembled the HSV alkaline exonuclease in many biochemical properties, and hybrid-arrested translation studies showed that it was virus coded, mapping at 0.080 to 0.185 genome map units. Exonuclease mRNA had a size and genome location equivalent to the mRNA encoding V185 in reticulocyte lysates, suggesting that V185 is the exonuclease. The enzyme synthesized in oocytes was found to act as an exonuclease in vivo. Two plasmids containing HSV DNA fragments directed the synthesis of exonuclease when microinjected into oocyte nuclei, and this finding enabled the coding and control sequences for this gene to be localized to 0.155 to 0.185 genome map units.

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Year:  1982        PMID： 6287023      PMCID： PMC256140     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  30 in total

1.  Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.

Authors:  J B Gurdon
Journal:  J Embryol Exp Morphol       Date:  1976-12

2.  The cell-free synthesis of herpesvirus-induced polypeptides.

Authors:  C M Preston
Journal:  Virology       Date:  1977-05-01       Impact factor: 3.616

3.  Cell-free synthesis of herpes simplex virus-coded pyrimidine deoxyribonucleoside kinase enzyme.

Authors:  C M Preston
Journal:  J Virol       Date:  1977-09       Impact factor: 5.103

4.  Analysis of herpesvirus DNA substructure by means of restriction endonucleases.

Authors:  J B Clements; R Cortini; N M Wilkie
Journal:  J Gen Virol       Date:  1976-02       Impact factor: 3.891

5.  Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins.

Authors:  R W Honess; B Roizman
Journal:  J Virol       Date:  1974-07       Impact factor: 5.103

6.  Biochemical studies on the herpes simplex virus-specified deoxypyrimidine kinase activity.

Authors:  A T Jamieson; J H Subak-Sharpe
Journal:  J Gen Virol       Date:  1974-09       Impact factor: 3.891

7.  A new DNA-exonuclease in cells infected with herpes virus: partial purification and properties of the enzyme.

Authors:  J M Morrison; H M Keir
Journal:  J Gen Virol       Date:  1968-12       Impact factor: 3.891

8.  Regulation of herpesvirus thymidine kinase activity in LM(TK) cells transformed by ultraviolet light-irradiated herpes simplex virus.

Authors:  S Kit; D R Dubbs
Journal:  Virology       Date:  1977-01       Impact factor: 3.616

9.  [125I]deoxycytidine used in a rapid, sensitive, and specific assay for herpes simplex virus type 1 thymidine kinase.

Authors:  W C Summers; W P Summers
Journal:  J Virol       Date:  1977-10       Impact factor: 5.103

10.  Herpes simplex virus gene expression in transformed cells. I. Regulation of the viral thymidine kinase gene in transformed L cells by products of superinfecting virus.

Authors:  J M Leiden; R Buttyan; P G Spear
Journal:  J Virol       Date:  1976-11       Impact factor: 5.103
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  14 in total

1.  Identification of herpes simplex virus type 1 genes required for origin-dependent DNA synthesis.

Authors:  C A Wu; N J Nelson; D J McGeoch; M D Challberg
Journal:  J Virol       Date:  1988-02       Impact factor: 5.103

2.  Isolation and characterization of herpes simplex virus type 1 host range mutants defective in viral DNA synthesis.

Authors:  E P Carmichael; M J Kosovsky; S K Weller
Journal:  J Virol       Date:  1988-01       Impact factor: 5.103

3.  A temperature-sensitive mutation in a herpes simplex virus type 1 gene required for viral DNA synthesis maps to coordinates 0.609 through 0.614 in UL.

Authors:  M E Marchetti; C A Smith; P A Schaffer
Journal:  J Virol       Date:  1988-03       Impact factor: 5.103

4.  DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes.

Authors:  D J McGeoch; A Dolan; M C Frame
Journal:  Nucleic Acids Res       Date:  1986-04-25       Impact factor: 16.971

5.  A method for identifying the viral genes required for herpesvirus DNA replication.

Authors:  M D Challberg
Journal:  Proc Natl Acad Sci U S A       Date:  1986-12       Impact factor: 11.205

6.  An ICP6::lacZ insertional mutagen is used to demonstrate that the UL52 gene of herpes simplex virus type 1 is required for virus growth and DNA synthesis.

Authors:  D J Goldstein; S K Weller
Journal:  J Virol       Date:  1988-08       Impact factor: 5.103

7.  High-resolution characterization of herpes simplex virus type 1 transcripts encoding alkaline exonuclease and a 50,000-dalton protein tentatively identified as a capsid protein.

Authors:  R H Costa; K G Draper; L Banks; K L Powell; G Cohen; R Eisenberg; E K Wagner
Journal:  J Virol       Date:  1983-12       Impact factor: 5.103

8.  Physical mapping of the herpes simplex virus type 2 nuc- lesion affecting alkaline exonuclease activity by using herpes simplex virus type 1 deletion clones.

Authors:  M W Wathen; J Hay
Journal:  J Virol       Date:  1984-07       Impact factor: 5.103

9.  Characterization of the herpes simplex virus type 1 glycoprotein D mRNA and expression of this protein in Xenopus oocytes.

Authors:  R J Watson; A M Colberg-Poley; C J Marcus-Sekura; B J Carter; L W Enquist
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

10.  Genetic analysis of temperature-sensitive mutants which define the genes for the major herpes simplex virus type 2 DNA-binding protein and a new late function.

Authors:  R A Dixon; D J Sabourin; P A Schaffer
Journal:  J Virol       Date:  1983-01       Impact factor: 5.103
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