Literature DB >> 198581

[125I]deoxycytidine used in a rapid, sensitive, and specific assay for herpes simplex virus type 1 thymidine kinase.

W C Summers, W P Summers.   

Abstract

[125I]deoxycytidine was a good substrate for herpes simplex virus type 1 thymidine kinase (TK), whereas [125I]deoxycytidine was a very poor substrate for cellular TK. Simple, sensitive, and specific assays for viral TK could be carried out in vivo and in vitro even in the presence of cell TK. Autoradiographic detection of incorporated [125I]deoxycytidine provided a rapid and simple method for detection of and screening for viral TK in infected as well as viral TK-transformed cells.

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Year:  1977        PMID： 198581      PMCID： PMC515932     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  10 in total

1.  MUTANT STRAINS OF HERPES SIMPLEX DEFICIENT IN THYMIDINE KINASE-INDUCING ACTIVITY.

Authors:  D R DUBBS; S KIT
Journal:  Virology       Date:  1964-04       Impact factor: 3.616

2.  Possible peptide chain termination mutants in thymide kinase gene of a mammalian virus, herpes simplex virus.

Authors:  W P Summers; M Wagner; W C Summers
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

3.  Absence of a requirement for host polypeptides in the herpes virus thymidine kinase.

Authors:  R W Honess; D H Watson
Journal:  J Gen Virol       Date:  1974-07       Impact factor: 3.891

4.  Phosphorylation of 5-bromodeoxycytidine in cells infected with herpes simplex virus.

Authors:  G M Cooper
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

5.  A virus-specific thymidine kinase in BHK-21 cells infected with herpes simplex virus.

Authors:  H G Klemperer; G R Haynes; W I Shedden; D H Watson
Journal:  Virology       Date:  1967-01       Impact factor: 3.616

6.  Gel electrophoresis and isoelectric focusing of mitochondrial and viral-induced thymidine kinases.

Authors:  S Kit; W C Leung; D Trkula; G Jorgensen
Journal:  Int J Cancer       Date:  1974-02-15       Impact factor: 7.396

7.  Iodination of nucleic acids in vitro.

Authors:  S L Commerford
Journal:  Biochemistry       Date:  1971-05-25       Impact factor: 3.162

8.  Studies of the enzymatic deamination of ara-cytidine. V. inhibition in vitro and in vivo by tetrahydrouridine and other reduced pyrimidine nucleosides.

Authors:  G W Camiener
Journal:  Biochem Pharmacol       Date:  1968-09       Impact factor: 5.858

9.  Deoxythymidine kinase induced in HeLa TK- cells by herpes simplex virus type I and type II. II. Purification and characterization.

Authors:  Y C Cheng; M Ostrander
Journal:  J Biol Chem       Date:  1976-05-10       Impact factor: 5.157

10.  Transfer of thymidine kinase to thymidine kinaseless L cells by infection with ultraviolet-irradiated herpes simplex virus.

Authors:  W Munyon; E Kraiselburd; D Davis; J Mann
Journal:  J Virol       Date:  1971-06       Impact factor: 5.103
  10 in total
  31 in total

Review 1.  Slipping and sliding: frameshift mutations in herpes simplex virus thymidine kinase and drug-resistance.

Authors:  Anthony Griffiths
Journal:  Drug Resist Updat       Date:  2011-09-22       Impact factor: 18.500

2.  Molecular analysis of chromosome-mediated gene transfer.

Authors:  G A Scangos; K M Huttner; S Silverstein; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

3.  Selective assay for herpes simplex viruses expressing thymidine kinase.

Authors:  J Campione-Piccardo; W E Rawls; S Bacchetti
Journal:  J Virol       Date:  1979-08       Impact factor: 5.103

4.  In vitro suppression of UAG and UGA mutants in the thymidine kinase gene of herpes simplex virus.

Authors:  K J Cremer; M Bodemer; W P Summers; W C Summers; R F Gesteland
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

5.  Characterization of the mRNA for herpes simplex virus thymidine kinase by cell-free synthesis of active enzyme.

Authors:  K Cremer; M Bodemer; W C Summers
Journal:  Nucleic Acids Res       Date:  1978-07       Impact factor: 16.971

6.  Comparative methods for detection of thymidine kinase-deficient herpes simplex virus type 1 strains.

Authors:  J Harmenberg; V A Sundqvist; H Gadler; B Levén; G Brännström; B Wahren
Journal:  Antimicrob Agents Chemother       Date:  1986-10       Impact factor: 5.191

7.  Replication and expression of thymidine kinase and human globin genes microinjected into mouse fibroblasts.

Authors:  W F Anderson; L Killos; L Sanders-Haigh; P J Kretschmer; E G Diacumakos
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

8.  Expression of the herpes thymidine kinase gene in Xenopus laevis oocytes: an assay for the study of deletion mutants constructed in vitro.

Authors:  S L McKnight; E R Gavis
Journal:  Nucleic Acids Res       Date:  1980-12-20       Impact factor: 16.971

9.  Vaccinia virus: a selectable eukaryotic cloning and expression vector.

Authors:  M Mackett; G L Smith; B Moss
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

10.  Genetic and biochemical characterization of the thymidine kinase gene from herpesvirus of turkeys.

Authors:  S L Martin; D I Aparisio; P K Bandyopadhyay
Journal:  J Virol       Date:  1989-06       Impact factor: 5.103
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