Literature DB >> 6280156

Expression in Escherichia coli of chemically synthesized gene for a novel opiate peptide alpha-neo-endorphin.

S Tanaka, T Oshima, K Ohsue, T Ono, S Oikawa, I Takano, T Noguchi, K Kangawa, N Minamino, H Matsuo.   

Abstract

Chemically synthesized alpha-neo-endorphin gene was fused to the Escherichia coli beta-galactosidase gene on the plasmid pKO13. The resulting recombinant DNA was used to transform E. coli cells. Radioimmunoassay for alpha-neo-endorphin in CNBr-treated bacterial cells showed that alpha-neo-endorphin was synthesized at approximately 5 x 10(5) molecules per single E. coli cell. One of the transformants, WA802/p alpha NE2, was used for alpha-neo-endorphin purification. From 10.9 g of wet cells, we isolated 4 mg of chemically pure and biologically active alpha-neo-endorphin.

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Year:  1982        PMID： 6280156      PMCID： PMC320563          DOI： 10.1093/nar/10.5.1741

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

1.  Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor.

Authors:  S Nakanishi; A Inoue; T Kita; M Nakamura; A C Chang; S N Cohen; S Numa
Journal:  Nature       Date:  1979-03-29       Impact factor: 49.962

2.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

3.  Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone.

Authors:  D V Goeddel; H L Heyneker; T Hozumi; R Arentzen; K Itakura; D G Yansura; M J Ross; G Miozzari; R Crea; P H Seeburg
Journal:  Nature       Date:  1979-10-18       Impact factor: 49.962

4.  alpha-Neo-endorphin : a "big" Leu-enkephalin with potent opiate activity from porcine hypothalami.

Authors:  K Kangawa; H Matsuo
Journal:  Biochem Biophys Res Commun       Date:  1979-01-15       Impact factor: 3.575

5.  Expression in Escherichia coli of chemically synthesized genes for human insulin.

Authors:  D V Goeddel; D G Kleid; F Bolivar; H L Heyneker; D G Yansura; R Crea; T Hirose; A Kraszewski; K Itakura; A D Riggs
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

6.  The complete amino acid sequence of alpha-neo-endorphin.

Authors:  K Kangawa; N Minamino; N Chino; S Sakakibara; H Matsuo
Journal:  Biochem Biophys Res Commun       Date:  1981-04-15       Impact factor: 3.575

7.  DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

Authors:  E Jay; R Bambara; R Padmanabhan; R Wu
Journal:  Nucleic Acids Res       Date:  1974-03       Impact factor: 16.971

8.  Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.

Authors:  K Itakura; T Hirose; R Crea; A D Riggs; H L Heyneker; F Bolivar; H W Boyer
Journal:  Science       Date:  1977-12-09       Impact factor: 47.728

9.  A plasmid cloning vehicle allowing regulated expression of eukaryotic DNA in bacteria.

Authors:  B Polisky; R J Bishop; D H Gelfand
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

10.  The origin of acetylcholine released from guinea-pig intestine and longitudinal muscle strips.

Authors:  W D Paton; M A Zar
Journal:  J Physiol       Date:  1968-01       Impact factor: 5.182
  10 in total
  5 in total

1.  A comprehensive list of chemically synthesized genes.

Authors:  G Gröger; F Ramalho-Ortigao; H Steil; H Seliger
Journal:  Nucleic Acids Res       Date:  1988-08-25       Impact factor: 16.971

2.  Expression of chemically synthesized alpha-neo-endorphin gene fused to E. coli alkaline phosphatase.

Authors:  K Ohsuye; M Nomura; S Tanaka; I Kubota; H Nakazato; H Shinagawa; A Nakata; T Noguchi
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

Review 3.  Recent developments in the chemical synthesis of polynucleotides.

Authors:  E Ohtsuka; M Ikehara; D Söll
Journal:  Nucleic Acids Res       Date:  1982-11-11       Impact factor: 16.971

4.  Expression in Escherichia coli of chemically synthesized gene for the human immune interferon.

Authors:  S Tanaka; T Oshima; K Ohsuye; T Ono; A Mizono; A Ueno; H Nakazato; M Tsujimoto; N Higashi; T Noguchi
Journal:  Nucleic Acids Res       Date:  1983-03-25       Impact factor: 16.971

5.  The nucleotide sequence of the yeast PHO5 gene: a putative precursor of repressible acid phosphatase contains a signal peptide.

Authors:  K Arima; T Oshima; I Kubota; N Nakamura; T Mizunaga; A Toh-e
Journal:  Nucleic Acids Res       Date:  1983-03-25       Impact factor: 16.971
  5 in total

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