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Literature DB >> 286319

DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.

M Wigler, A Pellicer, S Silverstein, R Axel, G Urlaub, L Chasin.

Abstract

In this report, we demonstrate the feasibility of transforming mouse cells deficient in adenine phosphoribosyltransferase (aprt; AMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.7) to the aprt+ phenotype by means of DNA-mediated gene transfer. Transformation was effected by using unfractionated high molecular weight genomic DNA from Chinese hamster, human, and mouse cells and restriction endonuclease-digested DNA from rabbit liver. The transformation frequency observed was between 1 and 10 colonies per 10(6) cells per 20 microgram of donor DNA. Transformants displayed enzymatic activity that was donor derived as demonstrated by isoelectric focusing of cytoplasmic extracts. These transformants fall into two classes: those that are phenotypically stable when grown in the absence of selective pressure and those that are phenotypically unstable under the same conditions.

Entities: Gene Species

Mesh：

Substances：

Year: 1979 PMID： 286319 PMCID： PMC383253 DOI： 10.1073/pnas.76.3.1373

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

13 in total

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2. Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.

Authors: M Wigler; S Silverstein; L S Lee; A Pellicer; Y c Cheng; R Axel
Journal: Cell Date: 1977-05 Impact factor: 41.582

3. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.

Authors: M Wigler; A Pellicer; S Silverstein; R Axel
Journal: Cell Date: 1978-07 Impact factor: 41.582

4. Mutations affecting adenine phosphoribosyl transferase activity in Chinese hamster cells.

Authors: L A Chasin
Journal: Cell Date: 1974-05 Impact factor: 41.582

5. Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.

Authors: J H McCutchan; J S Pagano
Journal: J Natl Cancer Inst Date: 1968-08 Impact factor: 13.506

6. Cotransfer of two linked human genes into cultured mouse cells.

Authors: K Willecke; R Lange; A Krüger; T Reber
Journal: Proc Natl Acad Sci U S A Date: 1976-04 Impact factor: 11.205

7. Mutant alleles for hypoxanthine phosphoriboxyltransferase: codominant expression, complementation, and segregation in hybrid Chinese hamster cells.

Authors: L A Chasin; G Urlaub
Journal: Somatic Cell Genet Date: 1976-09

8. Isolation and partial characterization of three methotrexate-resistant phenotypes from Chinese hamster ovary cells.

Authors: W F Flintoff; S V Davidson; L Siminovitch
Journal: Somatic Cell Genet Date: 1976-05

9. Transformation of yeast.

Authors: A Hinnen; J B Hicks; G R Fink
Journal: Proc Natl Acad Sci U S A Date: 1978-04 Impact factor: 11.205

10. Transfer of the human gene for hypoxanthine-guanine phosphoribosyltransferase via isolated human metaphase chromosomes into mouse L-cells.

Authors: K Willecke; F H Ruddle
Journal: Proc Natl Acad Sci U S A Date: 1975-05 Impact factor: 11.205

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