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Literature DB >> 2836094

Yeast/herpes simplex virus thymidine kinase gene fusions yield fusion proteins with thymidine kinase activity.

Abstract

Expression in Saccharomyces cerevisiae of the Herpes Simplex Virus type-1 (HSV-1) thymidine kinase gene was accomplished by the construction of a gene fusion between the TK and a yeast gene. The fusion of yeast DNA sequences (which include a promoter and DNA that codes for the amino terminus end of the yeast gene product) with the TK gene resulted in a protein fusion with thymidine kinase activity.

Entities: Species

Mesh：

Substances：
Thymidine Kinase

Year: 1985 PMID： 2836094 DOI： 10.1007/BF00381169

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

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References

22 in total

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Journal: Biochemistry Date: 1970-10-27 Impact factor: 3.162

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Authors: R J Watson; J Parker; N P Fiil; J G Flaks; J D Friesen
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Authors: S L McKnight; E R Gavis
Journal: Nucleic Acids Res Date: 1980-12-20 Impact factor: 16.971

10. High frequency recombination and the expression of genes cloned on chimeric yeast plasmids: Identification of a fragment of 2-μm circle essential for transformation.

Authors: J B McNeil; R K Storms; J D Friesen
Journal: Curr Genet Date: 1980-07 Impact factor: 3.886

Yeast/herpes simplex virus thymidine kinase gene fusions yield fusion proteins with thymidine kinase activity.

1. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

3. Thymidine kinase: evidence for its absence from Neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid.

4. New chromosomal location for structural genes of ribosomal proteins.

5. Expression of the herpes thymidine kinase gene in Xenopus laevis oocytes: an assay for the study of deletion mutants constructed in vitro.

6. Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.

7. Isolation and characterization of yeast mutants auxotrophic for 2'-deoxythymidine 5'-monophosphate.

8. Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae.

9. DNA sequencing with chain-terminating inhibitors.

10. High frequency recombination and the expression of genes cloned on chimeric yeast plasmids: Identification of a fragment of 2-μm circle essential for transformation.