Literature DB >> 6273795

Lac repressor - lac operator interaction. Circular dichroism study.

F Culard, J C Maurizot.   

Abstract

The interaction between lac repressor and a small operator DNA fragment have been examined by circular dichroism spectroscopy. The binding of lac repressor on the operator induces a conformation change of the DNA which is different from that observed upon non specific binding on non operator DNA. The CD titration curve indicates that the stoechiometry of interaction is complex. A two operators-one repressor complex was found. This result was confirmed by a gel filtration experiment.

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Year:  1981        PMID： 6273795      PMCID： PMC327508          DOI： 10.1093/nar/9.19.5175

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  36 in total

Review 1.  Repressors.

Authors:  S Bourgeois; M Pfahl
Journal:  Adv Protein Chem       Date:  1976

2.  Photochemical reactions of LAC repressor. Effects on inducer binding.

Authors:  M Charlier; F Culard; J C Maurizot; C Helene
Journal:  Biochem Biophys Res Commun       Date:  1977-01-24       Impact factor: 3.575

3.  The amino-acid sequence of lac repressor.

Authors:  K Beyreuther; K Adler; N Geisler; A Klemm
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

4.  Construction, isolation and implications of repressor-galactosidase - beta-galactosidase hybrid molecules.

Authors:  J Kania; B Müller-Hill
Journal:  Eur J Biochem       Date:  1977-10-03

Review 5.  Lac repressor and lac operator.

Authors:  B Müller-Hill
Journal:  Prog Biophys Mol Biol       Date:  1975       Impact factor: 3.667

6.  A general method for the purification of restriction enzymes.

Authors:  P J Greene; H L Heyneker; F Bolivar; R L Rodriguez; M C Betlach; A A Covarrubias; K Backman; D J Russel; R Tait; H W Boyer
Journal:  Nucleic Acids Res       Date:  1978-07       Impact factor: 16.971

7.  DNA binding characteristics of lactose repressor and the trypsin-resistant core repressor.

Authors:  R B O'Gorman; M Dunaway; K S Matthews
Journal:  J Biol Chem       Date:  1980-11-10       Impact factor: 5.157

8.  Hybrid tetramers of native and core lactose repressor protein. Assessment of operator and nonspecific DNA binding parameters and their relationship.

Authors:  M Dunaway; K S Matthews
Journal:  J Biol Chem       Date:  1980-11-10       Impact factor: 5.157

9.  Equilibrium binding of inducer to lac repressor.operator DNA complex.

Authors:  R B O'Gorman; J M Rosenberg; O B Kallai; R E Dickerson; K Itakura; A D Riggs; K S Matthews
Journal:  J Biol Chem       Date:  1980-11-10       Impact factor: 5.157

10.  Interaction of lac repressor with alternating poly d (A-T) and poly d (G-C). Circular dichroism studies.

Authors:  M Durand; J C Maurizot
Journal:  Biochimie       Date:  1980       Impact factor: 4.079
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  22 in total

1.  Radiolysis of lac repressor by gamma-rays and heavy ions: a two-hit model for protein inactivation.

Authors:  Michel Charlier; Séverine Eon; Edouard Sèche; Serge Bouffard; Françoise Culard; Mélanie Spotheim-Maurizot
Journal:  Biophys J       Date:  2002-05       Impact factor: 4.033

Review 2.  DNA looping.

Authors:  K S Matthews
Journal:  Microbiol Rev       Date:  1992-03

3.  Binding of small acid-soluble spore proteins from Bacillus subtilis changes the conformation of DNA from B to A.

Authors:  S C Mohr; N V Sokolov; C M He; P Setlow
Journal:  Proc Natl Acad Sci U S A       Date:  1991-01-01       Impact factor: 11.205

4.  Interaction of the tight-binding I12-X86 lac repressor with non operator DNA: salt dependence of complex formation.

Authors:  P Grebert; J C Maurizot
Journal:  Nucleic Acids Res       Date:  1986-08-26       Impact factor: 16.971

5.  Lac repressor-Lac operator complexes. Solution X-ray scattering and electrophoretic studies.

Authors:  F Culard; M Charlier; J C Maurizot; A Tardieu
Journal:  Eur Biophys J       Date:  1987       Impact factor: 1.733

6.  DNA conformational change in Gal repressor-operator complex: involvement of central G-C base pair(s) of dyad symmetry.

Authors:  R M Wartell; S Adhya
Journal:  Nucleic Acids Res       Date:  1988-12-23       Impact factor: 16.971

7.  High resolution mapping of E.coli transcription elongation complex in situ reveals protein interactions with the non-transcribed strand.

Authors:  M Guérin; M Leng; A R Rahmouni
Journal:  EMBO J       Date:  1996-10-01       Impact factor: 11.598

8.  Demonstration of two operator elements in gal: in vitro repressor binding studies.

Authors:  A Majumdar; S Adhya
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

9.  CAP binding to B and Z forms of DNA.

Authors:  M G Fried; H M Wu; D M Crothers
Journal:  Nucleic Acids Res       Date:  1983-04-25       Impact factor: 16.971

10.  1H NMR study of the interaction of bacteriophage lambda Cro protein with the OR3 operator. Evidence for a change of the conformation of the OR3 operator on binding.

Authors:  M P Kirpichnikov; K D Hahn; F Buck; H Rüterjans; B K Chernov; A V Kurochkin; K G Skryabin; A A Bayev
Journal:  Nucleic Acids Res       Date:  1984-04-25       Impact factor: 16.971
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