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Literature DB >> 6272224

DNA sequence of the rat growth hormone gene: location of the 5' terminus of the growth hormone mRNA and identification of an internal transposon-like element.

Abstract

The present communication describes the molecular cloning and DNA sequence determination of the rat growth hormone (rGH) gene. The rGH gene was cloned on an 11 kilobase EcoRI fragment of total rat DNA; it has four intervening sequences which correspond in position to those of the human growth hormone (hGH) gene. One of the intervening sequences in the rGH gene contains a possible transposable element: a 200 base pair direct repeat that is itself flanked by an exact 15 base pair direct repeat. The DNA sequence was used to estimate the location of the 5' end of the mature growth hormone mRNA. By S1 nuclease mapping it was located approximately 25 bases "downstream" from a TATAAA sequence presumed to play a role in initiation of transcription of the rGH gene.

Entities: Gene Species

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Year: 1981 PMID： 6272224 PMCID： PMC326828 DOI： 10.1093/nar/9.9.2087

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

26 in total

1. Preparative fractionation of DNA restriction fragments by reversed phase column chromatography.

Authors: S C Hardies; R D Wells
Journal: Proc Natl Acad Sci U S A Date: 1976-09 Impact factor: 11.205

2. Regulation of growth hormone messenger RNA by thyroid and glucocorticoid hormones.

Authors: J A Martial; J D Baxter; H M Goodman; P H Seeburg
Journal: Proc Natl Acad Sci U S A Date: 1977-05 Impact factor: 11.205

3. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

4. Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone.

Authors: P H Seeburg; J Shine; J A Martial; J D Baxter; H M Goodman
Journal: Nature Date: 1977-12-08 Impact factor: 49.962

5. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.

Authors: P Leder; D Tiemeier; L Enquist
Journal: Science Date: 1977-04-08 Impact factor: 47.728

6. Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites.

Authors: B Gronenborn; J Messing
Journal: Nature Date: 1978-03-23 Impact factor: 49.962

7. Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone.

Authors: A H Tashjian; Y Yasumura; L Levine; G H Sato; M L Parker
Journal: Endocrinology Date: 1968-02 Impact factor: 4.736

8. Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.

Authors: R L Pearson; J F Weiss; A D Kelmers
Journal: Biochim Biophys Acta Date: 1971-02-11

9. Rat insulin genes: construction of plasmids containing the coding sequences.

Authors: A Ullrich; J Shine; J Chirgwin; R Pictet; E Tischer; W J Rutter; H M Goodman
Journal: Science Date: 1977-06-17 Impact factor: 47.728

10. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

55 in total

1. Cloning and nucleotide sequence of a cDNA encoding the mink growth hormone.

Authors: K Shoji; E Ohara; M Watahiki; Y Yoneda
Journal: Nucleic Acids Res Date: 1990-11-11 Impact factor: 16.971

2. Nucleotide sequence of small polyadenylated B2 RNA.

Authors: D A Kramerov; S V Tillib; A P Ryskov; G P Georgiev
Journal: Nucleic Acids Res Date: 1985-09-25 Impact factor: 16.971

3. Negative regulation of the human epsilon-globin gene by transcriptional interference: role of an Alu repetitive element.

Authors: J Wu; G J Grindlay; P Bushel; L Mendelsohn; M Allan
Journal: Mol Cell Biol Date: 1990-03 Impact factor: 4.272

DNA sequence of the rat growth hormone gene: location of the 5' terminus of the growth hormone mRNA and identification of an internal transposon-like element.

1. Preparative fractionation of DNA restriction fragments by reversed phase column chromatography.

2. Regulation of growth hormone messenger RNA by thyroid and glucocorticoid hormones.

3. A new method for sequencing DNA.

4. Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone.

5. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.

6. Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites.

7. Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone.

8. Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.

9. Rat insulin genes: construction of plasmids containing the coding sequences.

10. DNA sequencing with chain-terminating inhibitors.

1. Cloning and nucleotide sequence of a cDNA encoding the mink growth hormone.

2. Nucleotide sequence of small polyadenylated B2 RNA.

3. Negative regulation of the human epsilon-globin gene by transcriptional interference: role of an Alu repetitive element.

4. In vitro transcription of RU, a middle repetitive element of the rat genome.

5. Novel restriction fragment length polymorphism of the growth hormone gene in inbred rats.

6. Ruminant globin gene structures suggest an evolutionary role for Alu-type repeats.

7. Structural analysis of the gene encoding human gastrin: the large intron contains an Alu sequence.

8. Human U1 RNA pseudogenes may be generated by both DNA- and RNA-mediated mechanisms.

9. Ubiquitous transposon-like repeats B1 and B2 of the mouse genome: B2 sequencing.

10. Rat prostatic steroid binding protein: characterisation of the Alu element upstream of the C3 genes.