Literature DB >> 322278

EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.

P Leder, D Tiemeier, L Enquist.   

Abstract

A derivative of bacteriophage lambda has been modified and tested together with an appropriate host system to meet the criteria of EK2 biologic containment for cloning DNA from higher organisms. In this report certain of the safety features are summarized and some of the tests carried out to confirm the containment properties of the vector are described. The cloning efficiency of this system, together with available gene purification and hybrid screening technology, indicate that it can be used to clone DNA fragments carrying specific, unique mammalian genes.

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Year:  1977        PMID： 322278     DOI： 10.1126/science.322278

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  186 in total

1.  Target joining of duplicated insertion sequence IS21 is assisted by IstB protein in vitro.

Authors:  S Schmid; B Berger; D Haas
Journal:  J Bacteriol       Date:  1999-04       Impact factor: 3.490

Review 2.  Transfection of Enterobacteriaceae and its applications.

Authors:  R Benzinger
Journal:  Microbiol Rev       Date:  1978-03

3.  Cloning of immunoglobulin kappa light chain genes from mouse liver and myeloma MOPC 173.

Authors:  M Steinmetz; H G Zachau; B Mach
Journal:  Nucleic Acids Res       Date:  1979-07-25       Impact factor: 16.971

4.  DNA clones containing mouse immunoglobulin kappa chain genes isolated by in vitro packaging into phage lambda coats.

Authors:  R Lenhard-Schuller; B Hohn; C Brack; M Hirama; S Tonegawa
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

5.  Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control. II. DNA fragment insertion at the vicinity of the lac UV5 promoter.

Authors:  P Charnay; A Louise; A Fritsch; D Perrin; P Tiollais
Journal:  Mol Gen Genet       Date:  1979-02-26

6.  Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRI restriction site.

Authors:  C Pourcel; C Marchal; A Louise; A Fritsch; P Tiollais
Journal:  Mol Gen Genet       Date:  1979-02-26

7.  Electron microscopic analysis of transcription: mapping of initiation sites and direction of transcription.

Authors:  C Brack
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205

8.  Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

Authors:  A C Chang; S N Cohen
Journal:  J Bacteriol       Date:  1978-06       Impact factor: 3.490

9.  Hemophilia B (factor IXSeattle 2) due to a single nucleotide deletion in the gene for factor IX.

Authors:  B G Schach; S Yoshitake; E W Davie
Journal:  J Clin Invest       Date:  1987-10       Impact factor: 14.808

10.  Regulation of Bacillus subtilis glutamate synthase genes by the nitrogen source.

Authors:  D E Bohannon; M S Rosenkrantz; A L Sonenshein
Journal:  J Bacteriol       Date:  1985-09       Impact factor: 3.490
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