Literature DB >> 6270058

Restriction map of corynebacteriophages beta c and beta vir and physical localization of the diphtheria tox operon.

J J Costa, J L Michel, R Rappuoli, J R Murphy.   

Abstract

The BamHI, EcoRI, HindIII, and KpnI restriction endonuclease maps of corynebacteriophage beta c and beta vir were constructed. beta vir appeared to be identical to beta c, except for an approximate 1-kilobase deletion that removed a BamHI site, two KpnI sites, and three EcoRI restriction sites. The diphtheria tox operon was located by hybridizing in vitro 32P-labeled tox messenger ribonucleic acid to blots of endonuclease-digested beta deoxyribonucleic acids. The messenger ribonucleic acid probe was found to hybridize to a 2.1-kilobase region of the beta genome. Since approximately 1.9 kilobases is required to encode prodiphtheria toxin, the data presented strongly suggest that the tox operon of beta is monocistronic.

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Year:  1981        PMID： 6270058      PMCID： PMC216174          DOI： 10.1128/jb.148.1.124-130.1981

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  29 in total

1.  Studies on the virulence of bacteriophage-infected strains of Corynebacterium diphtheriae.

Authors:  V J FREEMAN
Journal:  J Bacteriol       Date:  1951-06       Impact factor: 3.490

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.

Authors:  R B Helling; H M Goodman; H W Boyer
Journal:  J Virol       Date:  1974-11       Impact factor: 5.103

4.  Physical mapping of beta-converting and gamma-nonconverting corynebacteriophage genomes.

Authors:  G A Buck; N B Groman
Journal:  J Bacteriol       Date:  1981-10       Impact factor: 3.490

5.  Precursor in cotranslational secretion of diphtheria toxin.

Authors:  W P Smith; P C Tai; J R Murphy; B D Davis
Journal:  J Bacteriol       Date:  1980-01       Impact factor: 3.490

6.  Temperature-sensitive mutants of toxinogenic corynebacteriophage beta. I. Genetics.

Authors:  R A Singer
Journal:  Virology       Date:  1973-10       Impact factor: 3.616

7.  Evidence that the regulation of diphtheria toxin production is directed at the level of transcription.

Authors:  J R Murphy; J L Michel; M Teng
Journal:  J Bacteriol       Date:  1978-08       Impact factor: 3.490

8.  A membrane-filter technique for the detection of complementary DNA.

Authors:  D T Denhardt
Journal:  Biochem Biophys Res Commun       Date:  1966-06-13       Impact factor: 3.575

9.  Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch.

Authors:  R B Wallace; J Shaffer; R F Murphy; J Bonner; T Hirose; K Itakura
Journal:  Nucleic Acids Res       Date:  1979-08-10       Impact factor: 16.971

10.  Site specific enzymatic cleavage of RNA.

Authors:  H Donis-Keller
Journal:  Nucleic Acids Res       Date:  1979-09-11       Impact factor: 16.971
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  13 in total

1.  Genetic construction, expression, and melanoma-selective cytotoxicity of a diphtheria toxin-related alpha-melanocyte-stimulating hormone fusion protein.

Authors:  J R Murphy; W Bishai; M Borowski; A Miyanohara; J Boyd; S Nagle
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

2.  Identification of deoxyribonucleic acid restriction fragments of beta-converting corynebacteriophages that carry the gene for diphtheria toxin.

Authors:  G A Buck; N B Groman
Journal:  J Bacteriol       Date:  1981-10       Impact factor: 3.490

3.  The complete nucleotide sequence of the gene coding for diphtheria toxin in the corynephage omega (tox+) genome.

Authors:  G Ratti; R Rappuoli; G Giannini
Journal:  Nucleic Acids Res       Date:  1983-10-11       Impact factor: 16.971

4.  Molecular cloning and expression of gene fragments from corynebacteriophage beta encoding enzymatically active peptides of diphtheria toxin.

Authors:  R K Tweten; R J Collier
Journal:  J Bacteriol       Date:  1983-11       Impact factor: 3.490

5.  Restriction endonuclease map of corynebacteriophage omega ctox+ isolated from the Park-Williams no. 8 strain of Corynebacterium diphtheriae.

Authors:  R Rappuoli; J L Michel; J R Murphy
Journal:  J Virol       Date:  1983-02       Impact factor: 5.103

6.  DNA element of Corynebacterium diphtheriae with properties of an insertion sequence and usefulness for epidemiological studies.

Authors:  R Rappuoli; M Perugini; G Ratti
Journal:  J Bacteriol       Date:  1987-01       Impact factor: 3.490

7.  Molecular epidemiological studies of United States Gulf Coast Vibrio cholerae strains: integration site of mutator vibriophage VcA-3.

Authors:  S Goldberg; J R Murphy
Journal:  Infect Immun       Date:  1983-10       Impact factor: 3.441

8.  Characterization of the diphtheria tox transcript in Corynebacterium diphtheriae and Escherichia coli.

Authors:  D Leong; J R Murphy
Journal:  J Bacteriol       Date:  1985-09       Impact factor: 3.490

9.  Restriction endonuclease map of the nontoxigenic corynephage gamma c and its relationship to the toxigenic corynephage beta c.

Authors:  J L Michel; R Rappuoli; J R Murphy; A M Pappenheimer
Journal:  J Virol       Date:  1982-05       Impact factor: 5.103

10.  Detection and expression of DNA homologous to the tox gene in nontoxinogenic isolates of Corynebacterium diphtheriae.

Authors:  N Groman; N Cianciotto; M Bjorn; M Rabin
Journal:  Infect Immun       Date:  1983-10       Impact factor: 3.441
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