Literature DB >> 386279

Site specific enzymatic cleavage of RNA.

H Donis-Keller.   

Abstract

The hybridization of a DNA oligonucleotide a specific tetramer or longer) will direct a cleavage by RNase H (EC 3.1.4.34) to a specific site in RNA. The resulting fragments can then be labeled at their 5' or 3' ends, purified, and sequenced directly. This procedure is demonstrated with two RNA molecules of known sequence: 5.8S rRNA from yeast (158 nucleotides) and satellite tobacco necrosis virus (STNV) RNA (1240 nucleotides).

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Year:  1979        PMID： 386279      PMCID： PMC328004          DOI： 10.1093/nar/7.1.179

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  31 in total

1.  The use of nuclease P1 in sequence analysis of end group labeled RNA.

Authors:  M Silberklang; A M Gillum; U L RajBhandary
Journal:  Nucleic Acids Res       Date:  1977-12       Impact factor: 16.971

2.  Sequence analysis of 5'[32P] labeled mRNA and tRNA using polyacrylamide gel electrophoresis.

Authors:  R E Lockard; B Alzner-Deweerd; J E Heckman; J MacGee; M W Tabor; U L RajBhandary
Journal:  Nucleic Acids Res       Date:  1978-01       Impact factor: 16.971

3.  The sequence of Escherichia coli ribosomal 16 S RNA determined by new rapid gel methods.

Authors:  P Carbon; C Ehresmann; B Ehresmann; J P Ebel
Journal:  FEBS Lett       Date:  1978-10-01       Impact factor: 4.124

4.  pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

Authors:  J G Sutcliffe
Journal:  Nucleic Acids Res       Date:  1978-08       Impact factor: 16.971

5.  Direct chemical method for sequencing RNA.

Authors:  D A Peattie
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

6.  3'-terminal labelling of RNA with T4 RNA ligase.

Authors:  T E England; O C Uhlenbeck
Journal:  Nature       Date:  1978-10-12       Impact factor: 49.962

7.  A different approach to RNA sequencing.

Authors:  J Stanley; S Vassilenko
Journal:  Nature       Date:  1978-07-06       Impact factor: 49.962

8.  Mapping adenines, guanines, and pyrimidines in RNA.

Authors:  H Donis-Keller; A M Maxam; W Gilbert
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

9.  Thermodynamics of a stable yeast 5.8S rRNA hairpin helix.

Authors:  D Lightfoot
Journal:  Nucleic Acids Res       Date:  1978-10       Impact factor: 16.971

10.  Simplified method for purification of ribonuclease H from calf thymus.

Authors:  J G Stavrianopoulos; E Chargaff
Journal:  Proc Natl Acad Sci U S A       Date:  1978-09       Impact factor: 11.205
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  115 in total

1.  Seasonal dynamics of bacterioplankton community structure in a eutrophic lake as determined by 5S rRNA analysis.

Authors:  M G Höfle; H Haas; K Dominik
Journal:  Appl Environ Microbiol       Date:  1999-07       Impact factor: 4.792

2.  An exon that prevents transport of a mature mRNA.

Authors:  M A MacMorris; D A Zorio; T Blumenthal
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-30       Impact factor: 11.205

3.  A two-step strategy for constructing specifically self-subtracted cDNA libraries.

Authors:  Paolo Laveder; Cristiano De Pittà; Stefano Toppo; Giorgio Valle; Gerolamo Lanfranchi
Journal:  Nucleic Acids Res       Date:  2002-05-01       Impact factor: 16.971

4.  Antisense oligonucleotides selected by hybridisation to scanning arrays are effective reagents in vivo.

Authors:  M Sohail; H Hochegger; A Klotzbücher; R L Guellec; T Hunt; E M Southern
Journal:  Nucleic Acids Res       Date:  2001-05-15       Impact factor: 16.971

5.  RNA stem-loop enhanced expression of previously non-expressible genes.

Authors:  Michael Paulus; Martin Haslbeck; Manfred Watzele
Journal:  Nucleic Acids Res       Date:  2004-05-26       Impact factor: 16.971

6.  Analysis of the Xist RNA isoforms suggests two distinctly different forms of regulation.

Authors:  Mingchao Ma; William M Strauss
Journal:  Mamm Genome       Date:  2005-06       Impact factor: 2.957

7.  Probing the function of conserved RNA structures in the 30S subunit of Escherichia coli ribosomes.

Authors:  M Almehdi; Y S Yoo; H W Schaup
Journal:  Nucleic Acids Res       Date:  1991-12-25       Impact factor: 16.971

8.  Selective isolation and detailed analysis of intra-RNA cross-links induced in the large ribosomal subunit of E. coli: a model for the tertiary structure of the tRNA binding domain in 23S RNA.

Authors:  P Mitchell; M Osswald; D Schueler; R Brimacombe
Journal:  Nucleic Acids Res       Date:  1990-08-11       Impact factor: 16.971

9.  Oligonucleotides complementary to the Oxytricha nova telomerase RNA delineate the template domain and uncover a novel mode of primer utilization.

Authors:  M Melek; B T Davis; D E Shippen
Journal:  Mol Cell Biol       Date:  1994-12       Impact factor: 4.272

10.  Labeling the peptidyltransferase center of the Escherichia coli ribosome with photoreactive tRNA(Phe) derivatives containing azidoadenosine at the 3' end of the acceptor arm: a model of the tRNA-ribosome complex.

Authors:  J Wower; S S Hixson; R A Zimmermann
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205
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