Literature DB >> 3095831

Genetic construction, expression, and melanoma-selective cytotoxicity of a diphtheria toxin-related alpha-melanocyte-stimulating hormone fusion protein.

J R Murphy, W Bishai, M Borowski, A Miyanohara, J Boyd, S Nagle.   

Abstract

The structural gene for diphtheria toxin, tox, has been modified at its Sph I site by the introduction of an oligonucleotide linker encoding a unique Pst I restriction endonuclease site and a synthetic oligonucleotide encoding alpha-melanocyte-stimulating hormone (alpha-MSH). The resulting fusion gene directs the expression of a diphtheria toxin-related alpha-MSH hybrid protein in which the diphtheria toxin receptor-binding domain has been replaced with alpha-MSH sequences. The chimeric toxin has been partially purified from periplasmic extracts of recombinant Escherichia coli K-12 and has been found to be selectively toxic for alpha-MSH receptor-positive human malignant melanoma NEL-M1 cells in vitro.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3095831      PMCID： PMC386907          DOI： 10.1073/pnas.83.21.8258

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  40 in total

1.  Hormone-receptor interactions. The message sequence of alpha-melanotropin: demonstration of two active sites.

Authors:  A Eberle; R Schwyzer
Journal:  Clin Endocrinol (Oxf)       Date:  1976       Impact factor: 3.478

2.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

3.  Preparation of concanavalin A-ricin A-chain conjugate and its biologic activity against various cultured cells.

Authors:  T Yamaguchi; R Kato; M Beppu; T Terao; Y Inoue; Y Ikawa; T Osawa
Journal:  J Natl Cancer Inst       Date:  1979-06       Impact factor: 13.506

4.  A hybrid protein containing the toxic subunit of ricin and the cell-specific subunit of human chorionic gonadotropin. I. Synthesis and characterization.

Authors:  T N Oeltmann; E C Heath
Journal:  J Biol Chem       Date:  1979-02-25       Impact factor: 5.157

5.  Kinetics of adenosinediphosphoribosylation of elongation factor 2 in cells exposed to diphtheria toxin.

Authors:  M R Moynihan; A M Pappenheimer
Journal:  Infect Immun       Date:  1981-05       Impact factor: 3.441

6.  Inhibitory effect of ammonium chloride and chloroquine on the entry of the toxic lectin modeccin into HeLa cells.

Authors:  K Sandvig; S Olsnes; A Pihl
Journal:  Biochem Biophys Res Commun       Date:  1979-09-27       Impact factor: 3.575

7.  Epidermal growth factor-toxin A chain conjugates: EGF-ricin A is a potent toxin while EGF-diphtheria fragment A is nontoxic.

Authors:  D B Cawley; H R Herschman; D G Gilliland; R J Collier
Journal:  Cell       Date:  1980-11       Impact factor: 41.582

8.  Inhibition of diphtheria toxin degradation and cytotoxic action by chloroquine.

Authors:  S Leppla; R B Dorland; J L Middlebrook
Journal:  J Biol Chem       Date:  1980-03-25       Impact factor: 5.157

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Toxicity of diphtheria toxin-related proteins produced by suppression of nonsense mutations.

Authors:  P Bacha; J R Murphy; M Moynihan
Journal:  J Biol Chem       Date:  1980-11-25       Impact factor: 5.157
View more
  28 in total

1.  A recombinant single-chain immunotoxin composed of anti-Tac variable regions and a truncated diphtheria toxin.

Authors:  V K Chaudhary; M G Gallo; D J FitzGerald; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

2.  Cloning and expression in Escherichia coli of three fragments of diphtheria toxin truncated within fragment B.

Authors:  W R Bishai; A Miyanohara; J R Murphy
Journal:  J Bacteriol       Date:  1987-04       Impact factor: 3.490

3.  Analysis of the diphtheria tox promoter by site-directed mutagenesis.

Authors:  J Boyd; J R Murphy
Journal:  J Bacteriol       Date:  1988-12       Impact factor: 3.490

4.  High-level expression of a proteolytically sensitive diphtheria toxin fragment in Escherichia coli.

Authors:  W R Bishai; R Rappuoli; J R Murphy
Journal:  J Bacteriol       Date:  1987-11       Impact factor: 3.490

Review 5.  Malignant melanoma and melanocortin 1 receptor.

Authors:  A A Rosenkranz; T A Slastnikova; M O Durymanov; A S Sobolev
Journal:  Biochemistry (Mosc)       Date:  2013-11       Impact factor: 2.487

6.  Secretory expression of recombinant diphtheria toxin mutants in B. Subtilis.

Authors:  J Zhou; R Petracca
Journal:  J Tongji Med Univ       Date:  1999

7.  Melanotropin receptors demonstrated in situ in human melanoma.

Authors:  J B Tatro; M Atkins; J W Mier; S Hardarson; H Wolfe; T Smith; M L Entwistle; S Reichlin
Journal:  J Clin Invest       Date:  1990-06       Impact factor: 14.808

8.  Sensitivity of cancer cells to truncated diphtheria toxin.

Authors:  Yi Zhang; Wendy Schulte; Desmond Pink; Kyle Phipps; Andries Zijlstra; John D Lewis; David Morton Waisman
Journal:  PLoS One       Date:  2010-05-05       Impact factor: 3.240

9.  Diphtheria toxin-related cytokine fusion proteins: elongation factor 2 as a target for the treatment of neoplastic disease.

Authors:  J vanderSpek; L Cosenza; T Woodworth; J C Nichols; J R Murphy
Journal:  Mol Cell Biochem       Date:  1994-09       Impact factor: 3.396

10.  Interleukin 2-diphtheria toxin fusion protein can abolish cell-mediated immunity in vivo.

Authors:  V E Kelley; P Bacha; O Pankewycz; J C Nichols; J R Murphy; T B Strom
Journal:  Proc Natl Acad Sci U S A       Date:  1988-06       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.