Literature DB >> 6260747

Isolation of Bacillus subtilis genes from a charon 4A library.

E Ferrari, D J Henner, J A Hoch.   

Abstract

A library of Bacillus subtilis chromosomal deoxyribonucleic acid (DNA) was constructed, using lambda charon 4A as a cloning vector. Partially cleaved Bacillus subtilis DNA was prepared by partial methylation with EcoRI methylase, followed by complete EcoRI endonuclease digestion. More than 95% of the phage particles carried B. subtilis DNA inserts. When this library was screened for transforming activity, using competent cells, 70% of the genetic markers tested were found in a sample of 1,710 plaques. Cloned genetic loci were found to be about 100-fold more efficient in transforming activity than chromosomal DNA. Intact phage particles containing the pheA locus were found to be able to transform competent recipients with approximately the same efficiency as phage DNA. Transformation by intact particles was insensitive to deoxyribonuclease.

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Year:  1981        PMID： 6260747      PMCID： PMC217104          DOI： 10.1128/jb.146.1.430-432.1981

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  10 in total

1.  Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.

Authors:  M Thomas; R W Davis
Journal:  J Mol Biol       Date:  1975-01-25       Impact factor: 5.469

2.  REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

Authors:  C Anagnostopoulos; J Spizizen
Journal:  J Bacteriol       Date:  1961-05       Impact factor: 3.490

3.  Packaging recombinant DNA molecules into bacteriophage particles in vitro.

Authors:  B Hohn; K Murray
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205

4.  Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors:  W D Benton; R W Davis
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

5.  Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.

Authors:  F R Blattner; B G Williams; A E Blechl; K Denniston-Thompson; H E Faber; L Furlong; D J Grunwald; D O Kiefer; D D Moore; J W Schumm; E L Sheldon; O Smithies
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

6.  A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

Authors:  L Clarke; J Carbon
Journal:  Cell       Date:  1976-09       Impact factor: 41.582

7.  The isolation of structural genes from libraries of eucaryotic DNA.

Authors:  T Maniatis; R C Hardison; E Lacy; J Lauer; C O'Connell; D Quon; G K Sim; A Efstratiadis
Journal:  Cell       Date:  1978-10       Impact factor: 41.582

8.  Construction of a colony bank of E. coli containing hybrid plasmids representative of the Bacillus subtilis 168 genome. Expression of functions harbored by the recombinant plasmids in B. subtilis.

Authors:  G Rapoport; A Klier; A Billault; F Fargette; R Dedonder
Journal:  Mol Gen Genet       Date:  1979-10-03

9.  Restriction enzyme analysis of Bacillus subtilis ribosomal ribonucleic acid genes.

Authors:  C P Moran; K F Bott
Journal:  J Bacteriol       Date:  1979-10       Impact factor: 3.490

10.  Cloning of randomly sheared DNA fragments from a phi 105 lysogen of Bacillus subtilis identification of prophage-containing clones.

Authors:  K W Hutchison; H O Halvorson
Journal:  Gene       Date:  1980-02       Impact factor: 3.688
  10 in total
  36 in total

1.  Transcriptional control of the Bacillus subtilis spoIID gene.

Authors:  S Rong; M S Rosenkrantz; A L Sonenshein
Journal:  J Bacteriol       Date:  1986-03       Impact factor: 3.490

2.  Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.

Authors:  H Shimotsu; D J Henner
Journal:  J Bacteriol       Date:  1986-10       Impact factor: 3.490

Review 3.  Revised genetic linkage map of Bacillus subtilis.

Authors:  P J Piggot; J A Hoch
Journal:  Microbiol Rev       Date:  1985-06

4.  Nonrandom cosmid cloning and prophage SP beta homology near the replication terminus of the Bacillus subtilis chromosome.

Authors:  D B Rowe; T P Iismaa; R G Wake
Journal:  J Bacteriol       Date:  1986-07       Impact factor: 3.490

5.  Regulation of Bacillus subtilis glutamate synthase genes by the nitrogen source.

Authors:  D E Bohannon; M S Rosenkrantz; A L Sonenshein
Journal:  J Bacteriol       Date:  1985-09       Impact factor: 3.490

6.  Synthesis of sn-glycerol 3-phosphate, a key precursor of membrane lipids, in Bacillus subtilis.

Authors:  H R Morbidoni; D de Mendoza; J E Cronan
Journal:  J Bacteriol       Date:  1995-10       Impact factor: 3.490

7.  Cloning of sporulation gene spoIIG in Bacillus subtilis.

Authors:  H Ayaki; Y Kobayashi
Journal:  J Bacteriol       Date:  1984-05       Impact factor: 3.490

8.  Cloning the gyrA gene of Bacillus subtilis.

Authors:  M F Lampe; K F Bott
Journal:  Nucleic Acids Res       Date:  1984-08-10       Impact factor: 16.971

9.  Physical mapping of stable RNA genes in Bacillus subtilis using polymerase chain reaction amplification from a yeast artificial chromosome library.

Authors:  K Okamoto; P Serror; V Azevedo; B Vold
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

10.  Expression of the Bacillus subtilis glutamine synthetase gene in Escherichia coli.

Authors:  A L Gardner; A I Aronson
Journal:  J Bacteriol       Date:  1984-06       Impact factor: 3.490
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