| Literature DB >> 6766891 |
Abstract
A gene bank for Bacillus subtilis has been developed by cloning randomly sheared DNA fragments of a B. subtilis (phi 105) lysogen DNA in Escherichia coli employing the pMB9 plasmid vector. The DNA was inserted by the oligo(dA)-oligo(dT) method, and the average insert size of the cloned DNA was 7 kilobase pairs (kb). Three clones have been identified which carry DNA from the phi 105 prophage. None of these clones contain the phage-chromosome junction.Entities:
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Year: 1980 PMID: 6766891 DOI: 10.1016/0378-1119(80)90004-9
Source DB: PubMed Journal: Gene ISSN: 0378-1119 Impact factor: 3.688