Literature DB >> 115842

Restriction enzyme analysis of Bacillus subtilis ribosomal ribonucleic acid genes.

C P Moran, K F Bott.   

Abstract

The organization of the ribosomal ribonucleic acid (rRNA) genes (rDNA) of Bacillus subtilis was examined by cleaving the genome with several restriction endonucleases. The rDNA sequences were assayed by hybridization with purified radioactive rRNA's. Our interpretation of the resulting electrophoretic patterns is strengthened by an analysis of a fragment of B. subtilis rDNA cloned in Escherichia coli. The results indicated that there are eight rRNA operons in B. subtilis. Each operon contains one copy of the sequences coding for 16S, 23S, and 5S rRNA. The sequences coding for 5S rRNA were shown to be more closely linked to the 23S rRNA genes than to the 16S rRNA genes.

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Year:  1979        PMID： 115842      PMCID： PMC216784          DOI： 10.1128/jb.140.1.99-105.1979

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  17 in total

1.  Nucleotide sequence of 5 S ribosomal RNA precursor from Bacillus subtilis.

Authors:  M L Sogin; N R Pace
Journal:  J Biol Chem       Date:  1976-06-10       Impact factor: 5.157

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  The number of rRNA genes in Escherichia coli.

Authors:  A Kiss; B Sain; P Venetianer
Journal:  FEBS Lett       Date:  1977-07-01       Impact factor: 4.124

4.  T4 DNA polymerase.

Authors:  I R Lehman
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

5.  Electron microscope mapping of the distribution of ribosomal genes of the Bacillus subtilis chromosome.

Authors:  L T Chow; N Davidson
Journal:  J Mol Biol       Date:  1973-04-05       Impact factor: 5.469

6.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

7.  Two-dimensional restriction analysis of the Bacillus subtilis genome: gene purification and ribosomal ribonucleic acid gene organization.

Authors:  S S Potter; K F Bott; J E Newbold
Journal:  J Bacteriol       Date:  1977-01       Impact factor: 3.490

8.  Ribosomal RNA genes in Bacillus subtilis. Evidence for a cotranscription mechanism.

Authors:  B Zingales; W Colli
Journal:  Biochim Biophys Acta       Date:  1977-02-16

9.  Spliced segments at the 5' terminus of adenovirus 2 late mRNA.

Authors:  S M Berget; C Moore; P A Sharp
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205

10.  Nucleotide sequence of the rightward operator of phage lambda.

Authors:  T Maniatis; A Jeffrey; D G Kleid
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205
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  10 in total

1.  16S rRNA sequence indicates that plant-pathogenic mycoplasmalike organisms are evolutionarily distinct from animal mycoplasmas.

Authors:  P O Lim; B B Sears
Journal:  J Bacteriol       Date:  1989-11       Impact factor: 3.490

2.  Tn5-induced mutants of Azotobacter vinelandii affected in nitrogen fixation under Mo-deficient and Mo-sufficient conditions.

Authors:  R D Joerger; R Premakumar; P E Bishop
Journal:  J Bacteriol       Date:  1986-11       Impact factor: 3.490

3.  tRNA genes are found between 16S and 23S rRNA genes in Bacillus subtilis.

Authors:  K Loughney; E Lund; J E Dahlberg
Journal:  Nucleic Acids Res       Date:  1982-03-11       Impact factor: 16.971

4.  Genetic mapping of a linked cluster of ribosomal ribonucleic acid genes in Bacillus subtilis.

Authors:  F E Wilson; J A Hoch; K Bott
Journal:  J Bacteriol       Date:  1981-11       Impact factor: 3.490

5.  An RNA-DNA copolymer whose synthesis is correlated with the transcriptional requirement for chromosomal initiation in Bacillus subtilis contains ribosomal RNA sequences.

Authors:  S J Séror-Laurent; G Henckes
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

6.  Organization of transfer and ribosomal ribonucleic acid genes in Bacillus subtilis.

Authors:  C P Moran; K F Bott
Journal:  J Bacteriol       Date:  1979-11       Impact factor: 3.490

7.  Structure and organization of rRNA operons in the region of the replication origin of the Bacillus subtilis chromosome.

Authors:  N Ogasawara; S Moriya; H Yoshikawa
Journal:  Nucleic Acids Res       Date:  1983-09-24       Impact factor: 16.971

8.  Molecular cloning of nif DNA from Azotobacter vinelandii.

Authors:  P E Bishop; T M Rizzo; K F Bott
Journal:  J Bacteriol       Date:  1985-04       Impact factor: 3.490

9.  Isolation of an autonomously replicating DNA fragment from the region of defective bacteriophage PBSX of Bacillus subtilis.

Authors:  L M Anderson; H E Ruley; K F Bott
Journal:  J Bacteriol       Date:  1982-06       Impact factor: 3.490

10.  Isolation of Bacillus subtilis genes from a charon 4A library.

Authors:  E Ferrari; D J Henner; J A Hoch
Journal:  J Bacteriol       Date:  1981-04       Impact factor: 3.490
  10 in total

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