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Literature DB >> 333431

Packaging recombinant DNA molecules into bacteriophage particles in vitro.

Abstract

Recombinant phage genomes made in reactions with purified enzymes may be recovered directly by packaging into phage heads in vitro. The process is efficient and nonselective and offers containment in initial stages of handling recombinant DNA. Ligase [poly(deoxyribonucleotide):poly-(deoxyribonucleotide) ligase (AMP-forming), EC 6.5.1.1] reaction products can recombine with endogenous phage DNA during packaging, but UV-irradiation eliminates the biological activity of the endogenous DNA.

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Year: 1977 PMID： 333431 PMCID： PMC431522 DOI： 10.1073/pnas.74.8.3259

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

28 in total

1. Excision of prophage lambda in a cell-free system.

Authors: S Gottesman; M Gottesman
Journal: Proc Natl Acad Sci U S A Date: 1975-06 Impact factor: 11.205

2. Capsid transformation during packaging of bacteriophage lambdaDNA.

Authors: T Hohn; M Wurtz; B Hohn
Journal: Philos Trans R Soc Lond B Biol Sci Date: 1976-11-30 Impact factor: 6.237

Review 3. Restriction endonucleases in the analysis and restructuring of dna molecules.

Authors: D Nathans; H O Smith
Journal: Annu Rev Biochem Date: 1975 Impact factor: 23.643

4. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors: P A Sharp; B Sugden; J Sambrook
Journal: Biochemistry Date: 1973-07-31 Impact factor: 3.162

5. Studies of mouse mitochondrial DNA in Escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids.

Authors: A C Chang; R A Lansman; D A Clayton; S N Cohen
Journal: Cell Date: 1975-10 Impact factor: 41.582

6. In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase.

Authors: J R Cameron; S M Panasenko; I R Lehman; R W Davis
Journal: Proc Natl Acad Sci U S A Date: 1975-09 Impact factor: 11.205

7. Lambdoid phages that simplify the recovery of in vitro recombinants.

Authors: N E Murray; W J Brammar; K Murray
Journal: Mol Gen Genet Date: 1977-01-07

8. Functional expression of cloned yeast DNA in Escherichia coli.

Authors: B Ratzkin; J Carbon
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

9. In vitro packaging of a lambda Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1.

Authors: N Sternberg; D Tiemeier; L Enquist
Journal: Gene Date: 1977-05 Impact factor: 3.688

10. Replication and transcription of eukaryotic DNA in Escherichia coli.

Authors: J F Morrow; S N Cohen; A C Chang; H W Boyer; H M Goodman; R B Helling
Journal: Proc Natl Acad Sci U S A Date: 1974-05 Impact factor: 11.205

151 in total

1. Cloning and expression of a Ralstonia eutropha HF39 gene mediating indigo formation in Escherichia coli.

Authors: S Drewlo; C O Brämer; M Madkour; F Mayer; A Steinbüchel
Journal: Appl Environ Microbiol Date: 2001-04 Impact factor: 4.792

2. A study of the organisation of the ribosomal ribonucleic acid gene cluster of Neurospora crassa by means of restriction endonuclease analysis and cloning in bacteriophage lambda.

Authors: R A Cox; K Peden
Journal: Mol Gen Genet Date: 1979-07-02

3. The rat serum albumin gene: analysis of cloned sequences.

Authors: T D Sargent; J R Wu; J M Sala-Trepat; R B Wallace; A A Reyes; J Bonner
Journal: Proc Natl Acad Sci U S A Date: 1979-07 Impact factor: 11.205

4. Identification and cDNA cloning of a new nodule-specific gene, Nms-25 (nodulin-25) of Medicago sativa.

Authors: G B Kiss; E Vincze; Z Végh; G Tóth; J Soós
Journal: Plant Mol Biol Date: 1990-04 Impact factor: 4.076

5. Molecular cloning and expression of a proteinase gene from Lactococcus lactis subsp. cremoris H2 and construction of a new lactococcal vector pFX1.

Authors: F F Xu; L E Pearce; P L Yu
Journal: Arch Microbiol Date: 1990 Impact factor: 2.552

6. Gene for the RNA polymerase sigma subunit mapped in Salmonella typhimurium and Escherichia coli by cloning and deletion.

Authors: J G Scaife; J S Heilig; L Rowen; R Calendar
Journal: Proc Natl Acad Sci U S A Date: 1979-12 Impact factor: 11.205

10. Evolving sea urchin histone genes--nucleotide polymorphisms in the H4 gene and spacers of Strongylocentrotus purpuratus.

Authors: L N Yager; J F Kaumeyer; E S Weinberg
Journal: J Mol Evol Date: 1984 Impact factor: 2.395