Literature DB >> 6179043

Construction and expression in vivo of an internally deleted mouse alpha-fetoprotein gene: presence of a transcribed Alu-like repeat within the first intervening sequence.

P R Young, R W Scott, D H Hamer, S M Tilghman.   

Abstract

An alpha-fetoprotein (AFP) 'minigene' was constructed by joining the first two exons along with 0.9 kilobases of 5' flanking sequence of the mouse AFP gene to its last exon and 0.4 kilobases of 3' flanking sequence. This 'minigene' was tested for activity by inserting it into an SV40 vector and infecting African green monkey kidney cells. Correct initiation, termination, polyadenylation and splicing were observed. Furthermore a 220 nucleotide transcript was detected and mapped to a mouse Alu-like or B1 repeat on the opposite strand to that encoding the AFP gene in the first intervening sequence.

Entities:  

Mesh：

Substances：

Year:  1982        PMID： 6179043      PMCID： PMC320693          DOI： 10.1093/nar/10.10.3099

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  57 in total

1.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors:  M Grunstein; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.

Authors:  M Wigler; S Silverstein; L S Lee; A Pellicer; Y c Cheng; R Axel
Journal:  Cell       Date:  1977-05       Impact factor: 41.582

4.  Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors:  A J Berk; P A Sharp
Journal:  Cell       Date:  1977-11       Impact factor: 41.582

5.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

6.  On the location of palindromes in immunoglobulin genes.

Authors:  C Wuilmart; J Urbain; D Givol
Journal:  Proc Natl Acad Sci U S A       Date:  1977-06       Impact factor: 11.205

7.  Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors:  B Hirt
Journal:  J Mol Biol       Date:  1967-06-14       Impact factor: 5.469

8.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors:  H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

9.  Rat insulin genes: construction of plasmids containing the coding sequences.

Authors:  A Ullrich; J Shine; J Chirgwin; R Pictet; E Tischer; W J Rutter; H M Goodman
Journal:  Science       Date:  1977-06-17       Impact factor: 47.728

10.  Nucleotide sequence of the rightward operator of phage lambda.

Authors:  T Maniatis; A Jeffrey; D G Kleid
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205
View more
  25 in total

1.  Evolution of mouse B1 repeats: 7SL RNA folding pattern conserved.

Authors:  D Labuda; D Sinnett; C Richer; J M Deragon; G Striker
Journal:  J Mol Evol       Date:  1991-05       Impact factor: 2.395

2.  Nucleotide sequence of small polyadenylated B2 RNA.

Authors:  D A Kramerov; S V Tillib; A P Ryskov; G P Georgiev
Journal:  Nucleic Acids Res       Date:  1985-09-25       Impact factor: 16.971

3.  Negative regulation of the human epsilon-globin gene by transcriptional interference: role of an Alu repetitive element.

Authors:  J Wu; G J Grindlay; P Bushel; L Mendelsohn; M Allan
Journal:  Mol Cell Biol       Date:  1990-03       Impact factor: 4.272

4.  In vitro transcription of RU, a middle repetitive element of the rat genome.

Authors:  G Page; M Salvato; H M Goodman
Journal:  Nucleic Acids Res       Date:  1986-01-24       Impact factor: 16.971

5.  Fine-structure mapping of the three mouse alpha-fetoprotein gene enhancers.

Authors:  R Godbout; R S Ingram; S M Tilghman
Journal:  Mol Cell Biol       Date:  1988-03       Impact factor: 4.272

6.  Successive waves of fixation of B1 variants in rodent lineage history.

Authors:  Y Quentin
Journal:  J Mol Evol       Date:  1989-04       Impact factor: 2.395

7.  Two regulatory domains flank the mouse H19 gene.

Authors:  H Yoo-Warren; V Pachnis; R S Ingram; S M Tilghman
Journal:  Mol Cell Biol       Date:  1988-11       Impact factor: 4.272

8.  The organization of repetitive sequences in the albumin and alpha-fetoprotein gene loci in the rat.

Authors:  M Jose; I Tratner; M Poiret; J L Nahon; J L Danan; J M Sala-Trepat
Journal:  Mol Gen Genet       Date:  1989-01

9.  Multiple dispersed loci produce small cytoplasmic Alu RNA.

Authors:  R J Maraia; C T Driscoll; T Bilyeu; K Hsu; G J Darlington
Journal:  Mol Cell Biol       Date:  1993-07       Impact factor: 4.272

10.  The RNA polymerase III terminator used by a B1-Alu element can modulate 3' processing of the intermediate RNA product.

Authors:  R J Maraia; D Y Chang; A P Wolffe; R L Vorce; K Hsu
Journal:  Mol Cell Biol       Date:  1992-04       Impact factor: 4.272
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.