Literature DB >> 6938959

In vitro transcription of normal, mutant, and truncated mouse alpha-globin genes.

C A Talkington, Y Nishioka, P Leder.   

Abstract

We have transcribed various alpha-globin gene-containing templates by using a simplified in vitro system and have shown that the 5' end of each of the RNA polymerase II-specific transcripts corresponds to that of authentic alpha-globin mRNA. Using a truncated alpha-globin gene fragment, we then localized the promotor region of the adult alpha-globin gene to a 138-base-pair fragment of alpha-globin DNA, 141 nucleotides of which precede the initiation site. In contrast, when two naturally occurring alpha-globin gene mutants were tested as templates, no RNA polymerase II-dependent initiation was detected. Comparison of the nucleotide sequences of the normal and mutant alpha-globin genes has allowed us to focus upon nucleotide positions that might be essential for promoter activity.

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Year:  1980        PMID： 6938959      PMCID： PMC350455          DOI： 10.1073/pnas.77.12.7132

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  29 in total

1.  Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.

Authors:  D Shortle; D Nathans
Journal:  Proc Natl Acad Sci U S A       Date:  1978-05       Impact factor: 11.205

2.  Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors:  A J Berk; P A Sharp
Journal:  Cell       Date:  1977-11       Impact factor: 41.582

3.  pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

Authors:  J G Sutcliffe
Journal:  Nucleic Acids Res       Date:  1978-08       Impact factor: 16.971

Review 4.  Genetics of mouse hemoglobins.

Authors:  E S Russell; E C McFarland
Journal:  Ann N Y Acad Sci       Date:  1974-11-29       Impact factor: 5.691

5.  Studies on polynucleotides, C. A novel joining reaction catalyzed by the T4-polynucleotide ligase.

Authors:  V Sgaramella; J H Van de Sande; H G Khorana
Journal:  Proc Natl Acad Sci U S A       Date:  1970-11       Impact factor: 11.205

6.  Comparison of cloned mouse alpha- and beta-globin genes: conservation of intervening sequence locations and extragenic homology.

Authors:  A Leder; H I Miller; D H Hamer; J G Seidman; B Norman; M Sullivan; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

7.  Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

Authors:  G K McMaster; G G Carmichael
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

8.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

9.  Globin composition and synthesis of hemoglobins in developing fetal mice erythroid cells.

Authors:  A Fantoni; A Bank; P A Marks
Journal:  Science       Date:  1967-09-15       Impact factor: 47.728

10.  Purification using polyethylenimine precipitation and low molecular weight subunit analyses of calf thymus and wheat germ DNA-dependent RNA polymerase II.

Authors:  H G Hodo; S P Blatti
Journal:  Biochemistry       Date:  1977-05-31       Impact factor: 3.162
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  26 in total

1.  5-Methylcytidylic modification of in vitro transcript from the rat identifier sequence; evidence that the transcript forms a tRNA-like structure.

Authors:  K Sakamoto; N Okada
Journal:  Nucleic Acids Res       Date:  1985-10-25       Impact factor: 16.971

2.  Multiple 5'-flanking regions of the human alpha-skeletal actin gene synergistically modulate muscle-specific expression.

Authors:  G E Muscat; L Kedes
Journal:  Mol Cell Biol       Date:  1987-11       Impact factor: 4.272

3.  Tse-2: a trans-dominant extinguisher of albumin gene expression in hepatoma hybrid cells.

Authors:  A C Chin; R E Fournier
Journal:  Mol Cell Biol       Date:  1989-09       Impact factor: 4.272

4.  Total DNA transcription in vitro: a procedure to detect highly repetitive and transcribable sequences with tRNA-like structures.

Authors:  H Endoh; N Okada
Journal:  Proc Natl Acad Sci U S A       Date:  1986-01       Impact factor: 11.205

5.  Processing of mammalian tRNA transcripts in vitro: different pre-tRNAs are processed along alternative pathways that contain a common rate-limiting step.

Authors:  R J Rooney; J D Harding
Journal:  Nucleic Acids Res       Date:  1986-06-25       Impact factor: 16.971

6.  DNA sequences required for specific and efficient initiation of transcription at the polyoma virus early promoter.

Authors:  P Jat; U Novak; A Cowie; C Tyndall; R Kamen
Journal:  Mol Cell Biol       Date:  1982-07       Impact factor: 4.272

7.  Autoantibody reactive with three classes of RNA polymerases in sera from patients with systemic sclerosis.

Authors:  M Kuwana; J Kaburaki; T Mimori; T Tojo; M Homma
Journal:  J Clin Invest       Date:  1993-04       Impact factor: 14.808

8.  Formation of the transcription initiation complex on mammalian rDNA.

Authors:  H Kato; M Nagamine; R Kominami; M Muramatsu
Journal:  Mol Cell Biol       Date:  1986-10       Impact factor: 4.272

9.  Simian virus 40 major late promoter: an upstream DNA sequence required for efficient in vitro transcription.

Authors:  J Brady; M Radonovich; M Thoren; G Das; N P Salzman
Journal:  Mol Cell Biol       Date:  1984-01       Impact factor: 4.272

10.  Specific transcription of preformed nucleoprotein complexes, containing the adenovirus major late promoter, with a chromatographic fraction containing RNA polymerase II.

Authors:  R J Hellwig; S N Sinha; S K Niyogi
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205
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