Literature DB >> 6250034

Expression of cloned beta-endorphin gene sequences by Escherichia coli.

J Shine, I Fettes, N C Lan, J L Roberts, J D Baxter.   

Abstract

DNA coding for the opiate peptide beta-endorphin has been cloned into bacterial plasmids in such a way as to direct the synthesis of a hybrid beta-galactosidase/beta-endorphin protein. This hybrid protein can readily be cleaved in vitro to release biologically active beta-endorphin.

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Year:  1980        PMID： 6250034     DOI： 10.1038/285456a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  12 in total

1.  Inntags: small self-structured epitopes for innocuous protein tagging.

Authors:  Maya V Georgieva; Galal Yahya; Laia Codó; Raúl Ortiz; Laura Teixidó; José Claros; Ricardo Jara; Mònica Jara; Antoni Iborra; Josep Lluís Gelpí; Carme Gallego; Modesto Orozco; Martí Aldea
Journal:  Nat Methods       Date:  2015-08-31       Impact factor: 28.547

Review 2.  The purification of eukaryotic polypeptides synthesized in Escherichia coli.

Authors:  F A Marston
Journal:  Biochem J       Date:  1986-11-15       Impact factor: 3.857

3.  [Prospects and limitations of genetic engineering].

Authors:  W Klingmüller
Journal:  Naturwissenschaften       Date:  1981-03

4.  Development of the National Institutes of Health Guidelines for Recombinant DNA Research.

Authors:  B Talbot
Journal:  Public Health Rep       Date:  1983 Jul-Aug       Impact factor: 2.792

5.  Expression of antigenic determinants of the hemagglutinin gene of a human influenza virus in Escherichia coli.

Authors:  A R Davis; D P Nayak; M Ueda; A L Hiti; D Dowbenko; D G Kleid
Journal:  Proc Natl Acad Sci U S A       Date:  1981-09       Impact factor: 11.205

6.  Amplification of maize ribulose bisphosphate carboxylase large subunit synthesis in E. coli by transcriptional fusion with the lambda N operon.

Authors:  A A Gatenby; J A Castleton
Journal:  Mol Gen Genet       Date:  1982

7.  Multiple joined genes prevent product degradation in Escherichia coli.

Authors:  S H Shen
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205

8.  Production in Escherichia coli of biologically active secretin, a gastrointestinal hormone.

Authors:  M Suzuki; S Sumi; A Hasegawa; T Nishizawa; K Miyoshi; S Wakisaka; T Miyake; F Misoka
Journal:  Proc Natl Acad Sci U S A       Date:  1982-04       Impact factor: 11.205

9.  Solubilization and immune-detection of beta-galactosidase hybrid proteins carrying foreign antigenic determinants.

Authors:  K K Stanley
Journal:  Nucleic Acids Res       Date:  1983-06-25       Impact factor: 16.971

10.  Recombinant DNA in medicine.

Authors:  S D Cederbaum; G C Fareed; M A Lovett; L J Shapiro
Journal:  West J Med       Date:  1984-08
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