Literature DB >> 26322837

Inntags: small self-structured epitopes for innocuous protein tagging.

Maya V Georgieva1, Galal Yahya1,2, Laia Codó3,4, Raúl Ortiz1, Laura Teixidó5, José Claros6, Ricardo Jara6, Mònica Jara5, Antoni Iborra5, Josep Lluís Gelpí3,4,7, Carme Gallego1, Modesto Orozco4,7,8, Martí Aldea1.   

Abstract

Protein tagging is widely used in approaches ranging from affinity purification to fluorescence-based detection in live cells. However, an intrinsic limitation of tagging is that the native function of the protein may be compromised or even abolished by the presence of the tag. Here we describe and characterize a set of small, innocuous protein tags (inntags) that we anticipate will find application in a variety of biological techniques.

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Year:  2015        PMID： 26322837     DOI： 10.1038/nmeth.3556

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  34 in total

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Journal:  Nat Methods       Date:  2005-02-17       Impact factor: 28.547

Review 2.  Making the most of affinity tags.

Authors:  David S Waugh
Journal:  Trends Biotechnol       Date:  2005-06       Impact factor: 19.536

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Authors:  Jikui Song; John L Markley
Journal:  Protein Pept Lett       Date:  2007       Impact factor: 1.890

4.  Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies.

Authors:  Xiaodong Zhang; Chunguang Guo; Yueting Chen; Hennady P Shulha; Michael P Schnetz; Thomas LaFramboise; Cynthia F Bartels; Sanford Markowitz; Zhiping Weng; Peter C Scacheri; Zhenghe Wang
Journal:  Nat Methods       Date:  2008-01-06       Impact factor: 28.547

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6.  Transforming growth factor beta-induced phosphorylation of Smad3 is required for growth inhibition and transcriptional induction in epithelial cells.

Authors:  X Liu; Y Sun; S N Constantinescu; E Karam; R A Weinberg; H F Lodish
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-30       Impact factor: 11.205

7.  The Cln3 cyclin is down-regulated by translational repression and degradation during the G1 arrest caused by nitrogen deprivation in budding yeast.

Authors:  C Gallego; E Garí; N Colomina; E Herrero; M Aldea
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Authors:  Brian D Slaughter; Joel W Schwartz; Rong Li
Journal:  Proc Natl Acad Sci U S A       Date:  2007-12-12       Impact factor: 11.205

9.  A Whi7-anchored loop controls the G1 Cdk-cyclin complex at start.

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Journal:  Plant Cell       Date:  2020-05-14       Impact factor: 11.277

2.  Variable Effects of C-Terminal Fusions on FLS2 Function: Not All Epitope Tags Are Created Equal.

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3.  Internal epitope tagging informed by relative lack of sequence conservation.

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Journal:  Sci Rep       Date:  2016-11-28       Impact factor: 4.379

4.  Chimeric proteins tagged with specific 3xHA cassettes may present instability and functional problems.

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Journal:  PLoS One       Date:  2017-08-11       Impact factor: 3.240

5.  Competition in the chaperone-client network subordinates cell-cycle entry to growth and stress.

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Journal:  Life Sci Alliance       Date:  2019-04-15

6.  The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.

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7.  Antagonistic and synergistic epigenetic modulation using orthologous CRISPR/dCas9-based modular system.

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8.  Identification of Genes and Metabolic Pathways Involved in Resin Yield in Masson Pine by Integrative Analysis of Transcriptome, Proteome and Biochemical Characteristics.

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9.  An extra dimension in protein tagging by quantifying universal proteotypic peptides using targeted proteomics.

Authors:  Giel Vandemoortele; An Staes; Giulia Gonnelli; Noortje Samyn; Delphine De Sutter; Elien Vandermarliere; Evy Timmerman; Kris Gevaert; Lennart Martens; Sven Eyckerman
Journal:  Sci Rep       Date:  2016-06-06       Impact factor: 4.379
  9 in total

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