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Literature DB >> 6220717

Transformation of Aspergillus nidulans by the orotidine-5'-phosphate decarboxylase gene of Neurospora crassa.

Abstract

Relief of an auxotrophic requirement for uridine in Aspergillus nidulans strain G191 has been achieved by transformation with a segment of Neurospora crassa DNA containing the corresponding gene coding for orotidine-5'-phosphate decarboxylase. The mitotic stability of such transformants suggests that the DNA has integrated into the genome. Southern hybridisation analysis of DNA isolated from transformants revealed the presence of pBR322 sequences which have integrated into the host genome along with the N. crassa DNA.

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Year: 1983 PMID： 6220717 DOI： 10.1016/0006-291x(83)91828-4

Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575

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Cited

85 in total

1. Development of a transformation system for the thermophilic fungus Talaromyces sp. CL240 based on the use of phleomycin resistance as a dominant selectable marker.

Authors: S Jain; H Durand; G Tiraby
Journal: Mol Gen Genet Date: 1992-09

2. Differential flux through the quinate and shikimate pathways. Implications for the channelling hypothesis.

Authors: H K Lamb; J P van den Hombergh; G H Newton; J D Moore; C F Roberts; A R Hawkins
Journal: Biochem J Date: 1992-05-15 Impact factor: 3.857

3. Isolation of a gene required for programmed initiation of development by Aspergillus nidulans.

Authors: T H Adams; W A Hide; L N Yager; B N Lee
Journal: Mol Cell Biol Date: 1992-09 Impact factor: 4.272

4. Sequence and centromere proximal location of a transformation enhancing fragment ans1 from Aspergillus nidulans.

Authors: D Cullen; L J Wilson; G L Grey; D J Henner; G Turner; D J Ballance
Journal: Nucleic Acids Res Date: 1987-11-25 Impact factor: 16.971

5. Sequence of the cloned pyr4 gene of Trichoderma reesei and its use as a homologous selectable marker for transformation.

Authors: J L Smith; F T Bayliss; M Ward
Journal: Curr Genet Date: 1991-01 Impact factor: 3.886

6. In vivo overproduction of the pentafunctional arom polypeptide in Aspergillus nidulans affects metabolic flux in the quinate pathway.

Authors: H K Lamb; C R Bagshaw; A R Hawkins
Journal: Mol Gen Genet Date: 1991-06

Transformation of Aspergillus nidulans by the orotidine-5'-phosphate decarboxylase gene of Neurospora crassa.

1. Development of a transformation system for the thermophilic fungus Talaromyces sp. CL240 based on the use of phleomycin resistance as a dominant selectable marker.

2. Differential flux through the quinate and shikimate pathways. Implications for the channelling hypothesis.

3. Isolation of a gene required for programmed initiation of development by Aspergillus nidulans.

4. Sequence and centromere proximal location of a transformation enhancing fragment ans1 from Aspergillus nidulans.

5. Sequence of the cloned pyr4 gene of Trichoderma reesei and its use as a homologous selectable marker for transformation.

6. In vivo overproduction of the pentafunctional arom polypeptide in Aspergillus nidulans affects metabolic flux in the quinate pathway.

7. Functional analysis of the endoxylanase B (xynB) promoter from Penicillium purpurogenum.

8. Genetic Transformation of an argB Mutant of Aspergillus oryzae.

9. The oliC3 gene of Aspergillus niger: isolation, sequence and use as a selectable marker for transformation.

10. Transformation of Aspergillus nidulans by using a trpC plasmid.