Literature DB >> 1406595

Development of a transformation system for the thermophilic fungus Talaromyces sp. CL240 based on the use of phleomycin resistance as a dominant selectable marker.

S Jain1, H Durand, G Tiraby.   

Abstract

A transformation system for the thermophilic cellulolytic fungus Talaromyces sp. CL240 has been developed, using the phleomycin resistance gene from Streptoalloteichus hindustanus (Sh ble) as a dominant selectable marker. The plasmids (pAN8-1 and pUT720) carrying the Sh ble gene under the control of the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter, allowed selection of phleomycin-resistant transformants. A new promoter sequence cloned from chromosomal DNA of Trichoderma reesei (pUT737) was also able to drive efficient expression of the Sh ble gene in Talaromyces sp. CL240, resulting in the selection of transformants that were highly resistant to phleomycin.

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Year:  1992        PMID： 1406595     DOI： 10.1007/bf00538710

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  20 in total

1.  Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.

Authors:  P J Punt; R P Oliver; M A Dingemanse; P H Pouwels; C A van den Hondel
Journal:  Gene       Date:  1987       Impact factor: 3.688

2.  Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance.

Authors:  D Drocourt; T Calmels; J P Reynes; M Baron; G Tiraby
Journal:  Nucleic Acids Res       Date:  1990-07-11       Impact factor: 16.971

3.  Transformation of Ustilago maydis by a plasmid containing yeast 2-micron DNA.

Authors:  G R Banks
Journal:  Curr Genet       Date:  1983-03       Impact factor: 3.886

4.  Transformation of Penicillium chrysogenum with selection for increased resistance to benomyl.

Authors:  T M Picknett; G Saunders
Journal:  FEMS Microbiol Lett       Date:  1989-07-15       Impact factor: 2.742

5.  Transformation of Aspergillus nidulans with a cloned, oligomycin-resistant ATP synthase subunit 9 gene.

Authors:  M Ward; B Wilkinson; G Turner
Journal:  Mol Gen Genet       Date:  1986-02

6.  Efficient cloning of genes of Neurospora crassa.

Authors:  S J Vollmer; C Yanofsky
Journal:  Proc Natl Acad Sci U S A       Date:  1986-07       Impact factor: 11.205

7.  Efficient transformation of Neurospora crassa by utilizing hybrid plasmid DNA.

Authors:  M E Case; M Schweizer; S R Kushner; N H Giles
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

Review 8.  Structure and action of bleomycin.

Authors:  H Umezawa
Journal:  Prog Biochem Pharmacol       Date:  1976

9.  Proteolytic events in the processing of secreted proteins in fungi.

Authors:  T P Calmels; F Martin; H Durand; G Tiraby
Journal:  J Biotechnol       Date:  1991-01       Impact factor: 3.307

10.  A high efficiency transformation system for the basidiomycete Ustilago violacea employing hygromycin resistance and lithium-acetate treatment.

Authors:  A K Bej; M H Perlin
Journal:  Gene       Date:  1989-08-01       Impact factor: 3.688
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  7 in total

1.  The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas.

Authors:  D R Stevens; J D Rochaix; S Purton
Journal:  Mol Gen Genet       Date:  1996-04-24

2.  The ShBle resistance determinant from Streptoalloteichus hindustanus is expressed in Haloferax volcanii and confers resistance to bleomycin.

Authors:  S D Nuttall; S E Deutschel; R A Irving; J A Serrano-Gomicia; M L Dyall-Smith
Journal:  Biochem J       Date:  2000-03-01       Impact factor: 3.857

Review 3.  Selectable genetic markers for nematode transgenesis.

Authors:  Rosina Giordano-Santini; Denis Dupuy
Journal:  Cell Mol Life Sci       Date:  2011-03-24       Impact factor: 9.261

4.  Winged helix transcription factor CPCR1 is involved in regulation of beta-lactam biosynthesis in the fungus Acremonium chrysogenum.

Authors:  Esther K Schmitt; Astrid Bunse; Danielle Janus; Birgit Hoff; Ernst Friedlin; Hubert Kürnsteiner; Ulrich Kück
Journal:  Eukaryot Cell       Date:  2004-02

5.  Potential and utilization of thermophiles and thermostable enzymes in biorefining.

Authors:  Pernilla Turner; Gashaw Mamo; Eva Nordberg Karlsson
Journal:  Microb Cell Fact       Date:  2007-03-15       Impact factor: 5.328

6.  MADS-Box Transcription Factor MadsA Regulates Dimorphic Transition, Conidiation, and Germination of Talaromyces marneffei.

Authors:  Qiangyi Wang; Minghao Du; Shuai Wang; Linxia Liu; Liming Xiao; Linqi Wang; Tong Li; Hui Zhuang; Ence Yang
Journal:  Front Microbiol       Date:  2018-08-07       Impact factor: 5.640

Review 7.  Regulating Strategies for Producing Carbohydrate Active Enzymes by Filamentous Fungal Cell Factories.

Authors:  Teng Zhang; Hu Liu; Bo Lv; Chun Li
Journal:  Front Bioeng Biotechnol       Date:  2020-07-08
  7 in total

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