Literature DB >> 16347669

Genetic Transformation of an argB Mutant of Aspergillus oryzae.

Y T Hahm1, C A Batt.   

Abstract

An argB mutant of Aspergillus oryzae NRRL 492 has been genetically transformed with the Aspergillus nidulans argB gene. Protoplasts were generated with a combination of Novozyme 234 and beta-glucuronidase and regenerated on sucrose-stabilized minimal medium without arginine as described for A. nidulans. A frequency of 5 to 10 transformants per mug of DNA was obtained; however, most transformants appeared abortive. The A. nidulans argB gene and vector sequences appeared to be integrated into the A. oryzae chromosome.

Entities:  

Year:  1988        PMID： 16347669      PMCID： PMC202705          DOI： 10.1128/aem.54.6.1610-1611.1988

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

1.  Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations.

Authors:  M J Hynes; C M Corrick; J A King
Journal:  Mol Cell Biol       Date:  1983-08       Impact factor: 4.272

2.  Development of a high-frequency transforming vector for Aspergillus nidulans.

Authors:  D J Ballance; G Turner
Journal:  Gene       Date:  1985       Impact factor: 3.688

3.  Transformation of Aspergillus nidulans with a cloned, oligomycin-resistant ATP synthase subunit 9 gene.

Authors:  M Ward; B Wilkinson; G Turner
Journal:  Mol Gen Genet       Date:  1986-02

4.  Transformation of Aspergillus nidulans by using a trpC plasmid.

Authors:  M M Yelton; J E Hamer; W E Timberlake
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

5.  Transformation by integration in Aspergillus nidulans.

Authors:  J Tilburn; C Scazzocchio; G G Taylor; J H Zabicky-Zissman; R A Lockington; R W Davies
Journal:  Gene       Date:  1983-12       Impact factor: 3.688

6.  Transformation of Aspergillus nidulans by the orotidine-5'-phosphate decarboxylase gene of Neurospora crassa.

Authors:  D J Ballance; F P Buxton; G Turner
Journal:  Biochem Biophys Res Commun       Date:  1983-04-15       Impact factor: 3.575

7.  Cloning an Aspergillus nidulans developmental gene by transformation.

Authors:  I L Johnstone; S G Hughes; A J Clutterbuck
Journal:  EMBO J       Date:  1985-05       Impact factor: 11.598

8.  Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans.

Authors:  J M Kelly; M J Hynes
Journal:  EMBO J       Date:  1985-02       Impact factor: 11.598
  8 in total
  3 in total

1.  A gene transfer system based on the homologous pyrG gene and efficient expression of bacterial genes in Aspergillus oryzae.

Authors:  Y M de Ruiter-Jacobs; M Broekhuijsen; S E Unkles; E I Campbell; J R Kinghorn; R Contreras; P H Pouwels; C A van den Hondel
Journal:  Curr Genet       Date:  1989-09       Impact factor: 3.886

2.  Development of a transformation system for Aspergillus sojae based on the Agrobacterium tumefaciens-mediated approach.

Authors:  Rodrigo Mora-Lugo; Judith Zimmermann; Amira M Rizk; Marcelo Fernandez-Lahore
Journal:  BMC Microbiol       Date:  2014-09-25       Impact factor: 3.605

Review 3.  Methods for genetic transformation of filamentous fungi.

Authors:  Dandan Li; Yu Tang; Jun Lin; Weiwen Cai
Journal:  Microb Cell Fact       Date:  2017-10-03       Impact factor: 5.328
  3 in total

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