Literature DB >> 6203403

Identification of genetic variants in erythrocyte lysate by two-dimensional gel electrophoresis.

B B Rosenblum, J V Neel, S M Hanash, J L Joseph, N Yew.   

Abstract

Two-dimensional gel electrophoresis followed by silver-staining has been employed to study 27 red cell lysates for genetic variation. Forty-six polypeptides selected without respect to variability were considered suitable for scoring. Only 23 of the total of 1,242 polypeptides could not be scored unambiguously. Of the remaining 1,219 polypeptides, 38 exhibited the combination of a normal and a variant polypeptide. All variants were present in either the father or the mother of the subjects. The observed index of heterozygosity was 3.1% +/- 0.5%.

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Year:  1984        PMID： 6203403      PMCID： PMC1684473     

Source DB:  PubMed          Journal:  Am J Hum Genet        ISSN： 0002-9297            Impact factor:   11.025


  21 in total

1.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

2.  The distribution of isoelectric points of human soluble proteins and in particular of the enzyme products of 88 human gene loci.

Authors:  R A Fisher; W Putt; H Harris
Journal:  Ann Hum Genet       Date:  1977-05       Impact factor: 1.670

3.  Genetic polymorphism in normal human fibroblasts as analyzed by two-dimensional polyacrylamide gel electrophoresis.

Authors:  K E Walton; D Styer; E I Gruenstein
Journal:  J Biol Chem       Date:  1979-08-25       Impact factor: 5.157

4.  Microheterogeneity of serum transferrin, haptoglobin and alpha 2 HS glycoprotein examined by high resolution two-dimensional electrophoresis.

Authors:  N L Anderson; N G Anderson
Journal:  Biochem Biophys Res Commun       Date:  1979-05-14       Impact factor: 3.575

5.  Separation of allelic variants by two-dimensional electrophoresis.

Authors:  L A Wanner; J V Neel; M H Meisler
Journal:  Am J Hum Genet       Date:  1982-03       Impact factor: 11.025

6.  Genetic analysis of human lymphocyte proteins by two-dimensional gel electrophoresis: 1. Detection of genetic variant polypeptides in PHA-stimulated peripheral blood lymphocytes.

Authors:  H Hamaguchi; A Ohta; R Mukai; T Yabe; M Yamada
Journal:  Hum Genet       Date:  1981       Impact factor: 4.132

7.  Lack of genic variation in the abundant proteins of human kidney.

Authors:  S C Smith; R R Racine; C H Langley
Journal:  Genetics       Date:  1980-12       Impact factor: 4.562

8.  Red cell proteins. I. Two-dimensional mapping of human erythrocyte lysate proteins.

Authors:  J J Edwards; N G Anderson; S L Nance; N L Anderson
Journal:  Blood       Date:  1979-06       Impact factor: 22.113

9.  Human heterozygosity: a new estimate.

Authors:  E H McConkey; B J Taylor; D Phan
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

10.  Human hypoxanthine-guanine phosphoribosyltransferase. Purification and characterization of mutant forms of the enzyme.

Authors:  J M Wilson; B W Baugher; L Landa; W N Kelley
Journal:  J Biol Chem       Date:  1981-10-25       Impact factor: 5.157
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  13 in total

1.  James V. Neel, M.D., Ph.D. (March 22, 1915-January 31, 2000): founder effect.

Authors:  K M Weiss; R H Ward
Journal:  Am J Hum Genet       Date:  2000-03       Impact factor: 11.025

2.  Identification of a serum protein polymorphism via two-dimensional electrophoresis. Family and population studies in two genetically isolated groups: North American Hutterites and Australian aborigines.

Authors:  H Harrison; K Miller; C Ober; S Refetoff; M Dick; S Elias
Journal:  Am J Hum Genet       Date:  1991-02       Impact factor: 11.025

3.  Average locus differences in mutability related to protein "class": a hypothesis.

Authors:  J V Neel
Journal:  Proc Natl Acad Sci U S A       Date:  1990-03       Impact factor: 11.205

4.  Heterozygosity and ethnic variation in Japanese platelet proteins.

Authors:  J Asakawa; J V Neel; N Takahashi; C Satoh; S Kaneoka; E Nishikori; M Fujita
Journal:  Hum Genet       Date:  1988-01       Impact factor: 4.132

Review 5.  A revised estimate of the amount of genetic variation in human proteins: implications for the distribution of DNA polymorphisms.

Authors:  J V Neel
Journal:  Am J Hum Genet       Date:  1984-09       Impact factor: 11.025

6.  Two-dimensional gel studies of genetic variation in the plasma proteins of Amerindians and Japanese.

Authors:  J Asakawa; N Takahashi; B B Rosenblum; J V Neel
Journal:  Hum Genet       Date:  1985       Impact factor: 4.132

7.  Comparisons of genetic variability detected among mouse blood proteins using one- and two-dimensional electrophoreses.

Authors:  T W Sands; K A Hill; M L Petras
Journal:  Biochem Genet       Date:  1990-10       Impact factor: 1.890

8.  Genetic variants detected among 106 lymphocyte polypeptides observed in two-dimensional gels.

Authors:  S M Hanash; L J Baier; D Welch; R Kuick; M Galteau
Journal:  Am J Hum Genet       Date:  1986-09       Impact factor: 11.025

9.  Twenty-seven protein polymorphisms by two-dimensional electrophoresis of serum, erythrocytes, and fibroblasts in two pedigrees.

Authors:  D Goldman; L R Goldin; P Rathnagiri; S J O'Brien; J A Egeland; C R Merril
Journal:  Am J Hum Genet       Date:  1985-09       Impact factor: 11.025

10.  Genetic analysis of thirty-three platelet polypeptides detected in two-dimensional polyacrylamide gels.

Authors:  S M Hanash; J V Neel; L J Baier; B B Rosenblum; W Niezgoda; D Markel
Journal:  Am J Hum Genet       Date:  1986-03       Impact factor: 11.025
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