Literature DB >> 6170943

Identification of the modified nucleotides produced by covalent photoaddition of hydroxymethyltrimethylpsoralen to RNA.

J P Bachellerie, J F Thompson, M R Wegnez, J E Hearst.   

Abstract

The reaction between RNA and 4'hydroxymethyl-4,5',8-trimethylpsoralen has been studied. Both natural RNA and synthetic RNAs were used. The base specificity of the reaction was found to be the same in natural RNA, homopolymers, and mononucleotides. Uridine was found to be the most reactive base in all cases. The kinetics of formation and reversal of monoadducts and crosslinks has been examined. Paper electrophoretic conditions are described which provide a separation of the monoaddition and crosslinked photoproducts. The relative and absolute amounts of monoadducts and crosslinks can be determined very accurately with this system. Paper electrophoresis provides good separations of the different photoproducts. The mobilities of the products are a simple function of their molecular weights and charges.

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Year:  1981        PMID： 6170943      PMCID： PMC326836          DOI： 10.1093/nar/9.9.2207

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  17 in total

1.  Psoralen-DNA photoreaction: controlled production of mono- and diadducts with nanosecond ultraviolet laser pulses.

Authors:  B H Johnston; M A Johnson; C B Moore; J E Hearst
Journal:  Science       Date:  1977-08-26       Impact factor: 47.728

2.  Cross-linking of DNA in situ as a probe for chromatin structure.

Authors:  C V Hanson; C K Shen; J E Hearst
Journal:  Science       Date:  1976-07-02       Impact factor: 47.728

3.  Photobinding of 8-methoxypsoralen to transfer RNA and 5-fluorouracil-enriched transfer RNA.

Authors:  C N Ou; P S Song
Journal:  Biochemistry       Date:  1978-03-21       Impact factor: 3.162

4.  Light-induced cross-linking of DNA in the presence of a furocoumarin (psoralen). Studies with phage lambda, Escherichia coli, and mouse leukemia cells.

Authors:  R S Cole
Journal:  Biochim Biophys Acta       Date:  1970-09-17

5.  Studies on the photo-C4-cyclo-addition reactions between skin-photosensitizing furocoumarins and nucleic acids.

Authors:  L Musajo; G Rodighiero
Journal:  Photochem Photobiol       Date:  1970-01       Impact factor: 3.421

6.  A two-dimensional fractionation procedure for radioactive nucleotides.

Authors:  F Sanger; G G Brownlee; B G Barrell
Journal:  J Mol Biol       Date:  1965-09       Impact factor: 5.469

7.  The photoaddition of trimethylpsoralen to Drosophila melanogaster nuclei: a probe for chromatin substructure.

Authors:  G P Wieshahn; J E Hyde; J E Hearst
Journal:  Biochemistry       Date:  1977-03-08       Impact factor: 3.162

8.  Photoreactions at 3655 Angstrom between pyrimidine bases and skin-photosensitizing furocoumarins.

Authors:  L Musajo; F Bordin; G Caporale; S Marciani; G Rigatti
Journal:  Photochem Photobiol       Date:  1967-10       Impact factor: 3.421

9.  Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNA.

Authors:  S T Isaacs; C K Shen; J E Hearst; H Rapoport
Journal:  Biochemistry       Date:  1977-03-22       Impact factor: 3.162

10.  Photosensitization of skin in vivo by furocoumarins (psoralens).

Authors:  M A Pathak; D M Krämer
Journal:  Biochim Biophys Acta       Date:  1969-11-19
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  9 in total

1.  The human immunodeficiency virus type 1 packaging signal and major splice donor region have a conserved stable secondary structure.

Authors:  G P Harrison; A M Lever
Journal:  J Virol       Date:  1992-07       Impact factor: 5.103

2.  Antibody-nucleic acid interactions. Antibodies to psoralen-modified RNA as probes of RNA structure.

Authors:  D J Hurt; K D Winestock; M L O'Connor; M I Johnston
Journal:  Nucleic Acids Res       Date:  1987-11-11       Impact factor: 16.971

Review 3.  Structure and function of ribosomal RNA.

Authors:  R Brimacombe; W Stiege
Journal:  Biochem J       Date:  1985-07-01       Impact factor: 3.857

4.  Interaction of psoralen-derivatized oligodeoxyribonucleoside methylphosphonates with synthetic DNA containing a promoter for T7 RNA polymerase.

Authors:  B L Lee; K R Blake; P S Miller
Journal:  Nucleic Acids Res       Date:  1988-11-25       Impact factor: 16.971

5.  Secondary structure model of the Mason-Pfizer monkey virus 5' leader sequence: identification of a structural motif common to a variety of retroviruses.

Authors:  G P Harrison; E Hunter; A M Lever
Journal:  J Virol       Date:  1995-04       Impact factor: 5.103

6.  Characterization of the effects on ovalbumin mRNA of aminomethyl-trimethylpsoralen photoreaction with hen oviduct mRNA.

Authors:  C D Liarakos; G Reinhart; R A Kopper; R P Maddox
Journal:  Nucleic Acids Res       Date:  1987-10-26       Impact factor: 16.971

7.  Identification of a site of psoralen crosslinking in E. coli 16S ribosomal RNA.

Authors:  S Turner; J F Thompson; J E Hearst; H F Noller
Journal:  Nucleic Acids Res       Date:  1982-05-11       Impact factor: 16.971

8.  Triplex formation by a psoralen-conjugated oligodeoxyribonucleotide containing the base analog 8-oxo-adenine.

Authors:  P S Miller; G Bi; S A Kipp; V Fok; R K DeLong
Journal:  Nucleic Acids Res       Date:  1996-02-15       Impact factor: 16.971

9.  4.5S RNA is encoded by hundreds of tandemly linked genes, has a short half-life, and is hydrogen bonded in vivo to poly(A)-terminated RNAs in the cytoplasm of cultured mouse cells.

Authors:  L O Schoeniger; W R Jelinek
Journal:  Mol Cell Biol       Date:  1986-05       Impact factor: 4.272
  9 in total

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