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Literature DB >> 1061154

Interferon: purification and initial characterization from human diploid cells.

Abstract

Interferon produced by human diploid fibroblast cells in culture has been purified approximately 5000-fold. The purified interferon, when analyzed by electrophoresis on polyacrylamide gel containing sodium dodecyl sulfate, contains only one polypeptide component of 20,000 molecular weight. The interferon activity comigrates with this polypeptide, indicating identity of the activity and the polypeptide. Oxidation of this polypeptide with periodic acid and subsequent staining with Fuchsin base indicates that it contains carbohydrate ans suggests that the human fibroblast interferon is a glycoprotein.

Entities: Chemical Species

Mesh：

Substances：
Glycoproteins
Interferons

Year: 1976 PMID： 1061154 PMCID： PMC335941 DOI： 10.1073/pnas.73.2.520

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

21 in total

1. Suppression of human interferon production by inhibitors of glycosylation.

Authors: E A Havell; J Vilcek; E Falcoff; B Berman
Journal: Virology Date: 1975-02 Impact factor: 3.616

2. Affinity chromatography of human leukocyte and diploid cell interferons on sepharose-bound antibodies.

Authors: K Berg; C A Ogburn; K Paucker; K E Mogensen; K Cantell
Journal: J Immunol Date: 1975-02 Impact factor: 5.422

3. METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS.

Authors: M RODBELL
Journal: J Biol Chem Date: 1964-02 Impact factor: 5.157

4. Hormone-producing cultures of adrenal and pituitary tumor origin.

Authors: V BUONASSISI; G SATO; A I COHEN
Journal: Proc Natl Acad Sci U S A Date: 1962-07-15 Impact factor: 11.205

5. Binding of hormone to tissue: the first step in polypeptide hormone action.

Authors: I Pastan; J Roth; V Macchia
Journal: Proc Natl Acad Sci U S A Date: 1966-12 Impact factor: 11.205

6. Heterogeneity of purified mouse interferons.

Authors: E Knight
Journal: J Biol Chem Date: 1975-06-10 Impact factor: 5.157

7. Induction of T-cell differentiation in vitro by thymin, a purified polypeptide hormone of the thymus.

Authors: R S Basch; G Goldstein
Journal: Proc Natl Acad Sci U S A Date: 1974-04 Impact factor: 11.205

8. Distinct molecular species of interferons.

Authors: W E Stewart
Journal: Virology Date: 1974-09 Impact factor: 3.616

9. Photometric analysis of proteins and peptides at 191-194 millimicrons.

Authors: M M Mayer; J A Miller
Journal: Anal Biochem Date: 1970-07 Impact factor: 3.365

10. Isoelectric components of mouse, human, and rabbit interferons.

Authors: D Stancek; M Gressnerová; K Paucker
Journal: Virology Date: 1970-08 Impact factor: 3.616

50 in total

Review 1. Antiviral activity of interferons.

Authors: R M Friedman
Journal: Bacteriol Rev Date: 1977-09

2. Elimination of size and charge heterogeneities of human leukocyte interferons by chemical cleavage.

Authors: W E Stewart; L S Lin; M Wiranowska-Stewart; K Cantell
Journal: Proc Natl Acad Sci U S A Date: 1977-10 Impact factor: 11.205

3. Purification and characterization of interferon-like antiviral protein derived from flatfish (Paralichthys olivaceus) lymphocytes immortalized by oncogenes.

Authors: T Tamai; S Shirahata; N Sato; S Kimura; M Nonaka; H Murakami
Journal: Cytotechnology Date: 1993 Impact factor: 2.058

Interferon: purification and initial characterization from human diploid cells.

1. Suppression of human interferon production by inhibitors of glycosylation.

2. Affinity chromatography of human leukocyte and diploid cell interferons on sepharose-bound antibodies.

3. METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS.

4. Hormone-producing cultures of adrenal and pituitary tumor origin.

5. Binding of hormone to tissue: the first step in polypeptide hormone action.

6. Heterogeneity of purified mouse interferons.

7. Induction of T-cell differentiation in vitro by thymin, a purified polypeptide hormone of the thymus.

8. Distinct molecular species of interferons.

9. Photometric analysis of proteins and peptides at 191-194 millimicrons.

10. Isoelectric components of mouse, human, and rabbit interferons.

Review 1. Antiviral activity of interferons.

2. Elimination of size and charge heterogeneities of human leukocyte interferons by chemical cleavage.

3. Purification and characterization of interferon-like antiviral protein derived from flatfish (Paralichthys olivaceus) lymphocytes immortalized by oncogenes.

4. Production of human lymphoblastoid interferon by Namalva cells.

5. Heterocomplexes of human interferon and immunoglobulins: formation and properties.

6. Stable and unstable forms of human fibroblast interferon.

7. Ernest (Pete) Knight Jr. (1932-2013). A remembrance to a great interferon scientist and colleague.

8. Differential expression of human interferon genes.

9. Radioimmunoassay for human beta interferon.

10. The reactivation of human interferons by guanidine thiocyanate.