Literature DB >> 6093094

In vivo mutagenesis by O6-methylguanine built into a unique site in a viral genome.

E L Loechler, C L Green, J M Essigmann.   

Abstract

The mutagenicity of O6-methylguanine (O6MeGua), a chemical carcinogen-DNA adduct, has been studied in vivo by using a single-stranded M13mp8 genome in which a single O6MeGua residue was positioned in the unique recognition site for the restriction endonuclease Pst I. Transformation of Escherichia coli MM294A cells with this vector gave progeny phage, of which 0.4% were mutated in their Pst I site. In a separate experiment, cellular levels of O6MeGua-DNA methyltransferase (an O6MeGua-repair protein) were depleted by treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) prior to viral DNA uptake. In these cells, the mutation frequency due to O6MeGua increased with increasing MNNG dose (the highest mutation frequency observed was 20%). DNA sequence analysis of 60 mutant genomes revealed that O6MeGua induced exclusively G-to-A transitions.

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Year:  1984        PMID： 6093094      PMCID： PMC391905          DOI： 10.1073/pnas.81.20.6271

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

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Authors:  P Robins; J Cairns
Journal:  Nature       Date:  1979-07-05       Impact factor: 49.962

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Authors:  L Samson; J Cairns
Journal:  Nature       Date:  1977-05-19       Impact factor: 49.962

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Authors:  P Kleihues; G P Margison
Journal:  J Natl Cancer Inst       Date:  1974-12       Impact factor: 13.506

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Authors:  L L Gerchman; D B Ludlum
Journal:  Biochim Biophys Acta       Date:  1973-05-10

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Authors:  A Loveless
Journal:  Nature       Date:  1969-07-12       Impact factor: 49.962

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Authors:  P F Schendel; P E Robins
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

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Authors:  P J Abbott; R Saffhill
Journal:  Biochim Biophys Acta       Date:  1979-03-28

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Authors:  J R Mehta; D B Ludlum
Journal:  Biochim Biophys Acta       Date:  1978-12-21

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

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Authors:  T Lindahl; B Demple; P Robins
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
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  141 in total

1.  Targeted recovery of mutations in Drosophila.

Authors:  A Bentley; B MacLennan; J Calvo; C R Dearolf
Journal:  Genetics       Date:  2000-11       Impact factor: 4.562

Review 2.  Evolutionary consequences of nonrandom damage and repair of chromatin domains.

Authors:  T Boulikas
Journal:  J Mol Evol       Date:  1992-08       Impact factor: 2.395

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Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

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Journal:  Mol Gen Genet       Date:  1991-06

Review 5.  Alkyltransferase-like proteins: molecular switches between DNA repair pathways.

Authors:  Julie L Tubbs; John A Tainer
Journal:  Cell Mol Life Sci       Date:  2010-05-26       Impact factor: 9.261

6.  Site-specific mutagenesis by O6-alkylguanines located in the chromosomes of mammalian cells: influence of the mammalian O6-alkylguanine-DNA alkyltransferase.

Authors:  K S Ellison; E Dogliotti; T D Connors; A K Basu; J M Essigmann
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

7.  Target-selected mutant screen by TILLING in Drosophila.

Authors:  Sylke Winkler; Anja Schwabedissen; Dana Backasch; Christian Bökel; Claudia Seidel; Stefanie Bönisch; Maximilian Fürthauer; Antje Kuhrs; Laura Cobreros; Michael Brand; Marcos González-Gaitán
Journal:  Genome Res       Date:  2005-05       Impact factor: 9.043

8.  Spontaneous mutation in the Escherichia coli lacI gene.

Authors:  R M Schaaper; R L Dunn
Journal:  Genetics       Date:  1991-10       Impact factor: 4.562

9.  Relating aromatic hydrocarbon-induced DNA adducts and c-H-ras mutations in mouse skin papillomas: the role of apurinic sites.

Authors:  D Chakravarti; J C Pelling; E L Cavalieri; E G Rogan
Journal:  Proc Natl Acad Sci U S A       Date:  1995-10-24       Impact factor: 11.205

10.  Mechanisms of Insertion of dCTP and dTTP Opposite the DNA Lesion O6-Methyl-2'-deoxyguanosine by Human DNA Polymerase η.

Authors:  Amitraj Patra; Qianqian Zhang; F Peter Guengerich; Martin Egli
Journal:  J Biol Chem       Date:  2016-09-30       Impact factor: 5.157
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