Literature DB >> 7753849

Assembly of functional Escherichia coli RNA polymerase containing beta subunit fragments.

K Severinov1, A Mustaev, E Severinova, I Bass, M Kashlev, R Landick, V Nikiforov, A Goldfarb, S A Darst.   

Abstract

The Escherichia coli rpoB gene, which codes for the 1342-residue beta subunit of RNA polymerase (RNAP), contains two dispensable regions centered around codons 300 and 1000. To test whether these regions demarcate domains of the RNAP beta subunit, fragments encoded by segments of rpoB flanking the dispensable regions were individually overexpressed and purified. We show that these beta-subunit polypeptide fragments, when added with purified recombinant beta', sigma, and alpha subunits of RNAP, reconstitute a functional enzyme in vitro. These results demonstrate that the beta subunit is composed of at least three distinct domains and open another avenue for in vitro studies of RNAP assembly and structure.

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Year:  1995        PMID： 7753849      PMCID： PMC41990          DOI： 10.1073/pnas.92.10.4591

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  50 in total

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Authors:  H Heumann; H Lederer; W Kammerer; P Palm; W Metzger; G Baer
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Authors:  J M Ahearn; M S Bartolomei; M L West; L J Cisek; J L Corden
Journal:  J Biol Chem       Date:  1987-08-05       Impact factor: 5.157

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4.  Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases.

Authors:  L A Allison; M Moyle; M Shales; C J Ingles
Journal:  Cell       Date:  1985-09       Impact factor: 41.582

5.  RNA polymerase II of Drosophila. Relation of its 140,000 Mr subunit to the beta subunit of Escherichia coli RNA polymerase.

Authors:  D Falkenburg; B Dworniczak; D M Faust; E K Bautz
Journal:  J Mol Biol       Date:  1987-06-20       Impact factor: 5.469

6.  Specific proteolysis of native alanine racemases from Salmonella typhimurium: identification of the cleavage site and characterization of the clipped two-domain proteins.

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7.  Spinach chloroplast rpoBC genes encode three subunits of the chloroplast RNA polymerase.

Authors:  G S Hudson; T A Holton; P R Whitfield; W Bottomley
Journal:  J Mol Biol       Date:  1988-04-20       Impact factor: 5.469

8.  Prokaryotic and eukaryotic RNA polymerases have homologous core subunits.

Authors:  D Sweetser; M Nonet; R A Young
Journal:  Proc Natl Acad Sci U S A       Date:  1987-03       Impact factor: 11.205

9.  Structure of the eukaryotic transcription apparatus: features of the gene for the largest subunit of Drosophila RNA polymerase II.

Authors:  J Biggs; L L Searles; A L Greenleaf
Journal:  Cell       Date:  1985-09       Impact factor: 41.582

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  9 in total

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3.  Determinants of RNA polymerase alpha subunit for interaction with beta, beta', and sigma subunits: hydroxyl-radical protein footprinting.

Authors:  T Heyduk; E Heyduk; K Severinov; H Tang; R H Ebright
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Review 4.  Information processing by RNA polymerase: recognition of regulatory signals during RNA chain elongation.

Authors:  R A Mooney; I Artsimovitch; R Landick
Journal:  J Bacteriol       Date:  1998-07       Impact factor: 3.490

5.  Mapping the sigma70 subunit contact sites on Escherichia coli RNA polymerase with a sigma70-conjugated chemical protease.

Authors:  J T Owens; R Miyake; K Murakami; A J Chmura; N Fujita; A Ishihama; C F Meares
Journal:  Proc Natl Acad Sci U S A       Date:  1998-05-26       Impact factor: 11.205

6.  Preferential interaction of the his pause RNA hairpin with RNA polymerase beta subunit residues 904-950 correlates with strong transcriptional pausing.

Authors:  D Wang; K Severinov; R Landick
Journal:  Proc Natl Acad Sci U S A       Date:  1997-08-05       Impact factor: 11.205

7.  Modular organization of the catalytic center of RNA polymerase.

Authors:  A Mustaev; M Kozlov; V Markovtsov; E Zaychikov; L Denissova; A Goldfarb
Journal:  Proc Natl Acad Sci U S A       Date:  1997-06-24       Impact factor: 11.205

8.  Insusceptibility of members of the class Mollicutes to rifampin: studies of the Spiroplasma citri RNA polymerase beta-subunit gene.

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9.  Static and kinetic site-specific protein-DNA photocrosslinking: analysis of bacterial transcription initiation complexes.

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  9 in total

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