Literature DB >> 5663808

Proton magnetic resonance of proteins fully deuterated except for 1H-leucine side chains.

H L Crespi, R M Rosenberg, J J Katz.   

Abstract

The fully deuterated proteins C-phycocyanin, C-phycoerythrin, and cytochrome c have been obtained by biosynthesis with the leucine side chains, and only the leucine side chains, of normal ((1)H) isotopic composition. In these (isotopic hybrid) proteins, proton magnetic resonance analysis shows that the (1)H-leucine side chains are in a variety of environments. During protein biosynthesis, the alpha hydrogen of leucine is exchanged with a hydrogen ((2)H) from the aqueous medium.

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Year:  1968        PMID： 5663808     DOI： 10.1126/science.161.3843.795

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  16 in total

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Journal:  J Biol Chem       Date:  2008-12-22       Impact factor: 5.157

3.  Alternate HMQC experiments for recording HN and HC-correlation spectra in proteins at high throughput.

Authors:  Luciano Mueller
Journal:  J Biomol NMR       Date:  2008-09-27       Impact factor: 2.835

Review 4.  Methyl groups as probes of supra-molecular structure, dynamics and function.

Authors:  Amy M Ruschak; Lewis E Kay
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Authors:  Sai Chaitanya Chiliveri; Mandar V Deshmukh
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6.  Stable isotope labeling approaches for NMR characterization of glycoproteins using eukaryotic expression systems.

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Journal:  J Biomol NMR       Date:  2018-02-28       Impact factor: 2.835

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Review 8.  Isotope labeling for solution and solid-state NMR spectroscopy of membrane proteins.

Authors:  Raffaello Verardi; Nathaniel J Traaseth; Larry R Masterson; Vitaly V Vostrikov; Gianluigi Veglia
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9.  1H NMR studies of deuterated ribonuclease HI selectively labeled with protonated amino acids.

Authors:  Y Oda; H Nakamura; T Yamazaki; K Nagayama; M Yoshida; S Kanaya; M Ikehara
Journal:  J Biomol NMR       Date:  1992-03       Impact factor: 2.835

10.  (2)H- and(13)C-labeled amino acids generated by obligate methylotrophs Biosynthesis and MS monitoring.

Authors:  E N Karnaukhova; O S Reshetova; S Y Semenov; D A Skladnev; Y D Tsygankov
Journal:  Amino Acids       Date:  1994-06       Impact factor: 3.520
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