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Literature DB >> 7145700

A yeast transcription system for the 5S rRNA gene.

Abstract

A cell-free extract of yeast nuclei that can specifically transcribe cloned yeast 5S rRNA genes has been developed. Optima for transcription of 5S rDNA were determined and conditions of extract preparation leading to reproducible activities and specificities established. The major in vitro product has the same size and oligonucleotide composition as in vivo 5S rRNA. The in vitro transcription extract does not transcribe yeast tRNA genes. The extract does increase the transcription of tRNA genes packaged in chromatin.

Entities: Chemical Species

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Year: 1982 PMID： 7145700 PMCID： PMC320867 DOI： 10.1093/nar/10.17.5223

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

39 in total

Review 1. Preparation of RNA and ribosomes from yeast.

Authors: G M Rubin
Journal: Methods Cell Biol Date: 1975 Impact factor: 1.441

2. Analysis of 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedure.

Authors: G Volckaert; W M Jou; W Fiers
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

3. Molecular structure of yeast RNA polymerase III: demonstration of the tripartite transcriptive system in lower eukaryotes.

Authors: P Valenzuela; G L Hager; F Weinberg; W J Rutter
Journal: Proc Natl Acad Sci U S A Date: 1976-04 Impact factor: 11.205

4. Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.

Authors: G I Bell; L J DeGennaro; D H Gelfand; R J Bishop; P Valenzuela; W J Rutter
Journal: J Biol Chem Date: 1977-11-25 Impact factor: 5.157

5. Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o.

Authors: H M Goodman; M V Olson; B D Hall
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

6. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors: D B Clewell; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1969-04 Impact factor: 11.205

7. Transcription in yeast: alpha-amanitin sensitivity and other properties which distinguish between RNA polymerases I and III.

Authors: L D Schultz; B D Hall
Journal: Proc Natl Acad Sci U S A Date: 1976-04 Impact factor: 11.205

8. Transformation of yeast.

Authors: A Hinnen; J B Hicks; G R Fink
Journal: Proc Natl Acad Sci U S A Date: 1978-04 Impact factor: 11.205

9. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors: F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal: Gene Date: 1977 Impact factor: 3.688

10. Ribosomal RNA genes of Saccharomyces cerevisiae. II. Physical map and nucleotide sequence of the 5 S ribosomal RNA gene and adjacent intergenic regions.

Authors: P Valenzuela; G I Bell; A Venegas; E T Sewell; F R Masiarz; L J DeGennaro; F Weinberg; W J Rutter
Journal: J Biol Chem Date: 1977-11-25 Impact factor: 5.157

4 in total