Literature DB >> 518134

Purification and characterization of pregnancy associated plasma protein A (PAPP-A).

P Bischof.   

Abstract

Pregnancy Associated Plasma Protein A (PAPP-A) has been isolated from late pregnancy plasma using ammonium sulphate precipitation, ion exchange chromatography, affinity chromatography on Concanavalin-A, gel filtration and negative affinity chromatography. It was found that PAPP-A is an alpha 2-glycoprotein of 750-820 000 MW, probably a dimer with each monomer being composed of 2 polypeptide chains of 218 000 MW. The amino acid composition as well as other physicochemical characteristics are similar to human alpha 2-macroglobulin. PAPP-A exhibits in vitro an inhibition of the activity of the complement system, of the caseinolytic activity of plasmin and possibly of the urokinase activation of plasminogen. The hypothesis that PAPP-A plays a role in the regulation of fibrinolysis during pregnancy is put forward.

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Year:  1979        PMID： 518134     DOI： 10.1007/bf02109920

Source DB:  PubMed          Journal:  Arch Gynecol        ISSN： 0170-9925


  16 in total

1.  Chromatographic determination of cystine as cysteic acid.

Authors:  E SCHRAM; S MOORE; E J BIGWOOD
Journal:  Biochem J       Date:  1954-05       Impact factor: 3.857

2.  The fibrin plate method for estimating fibrinolytic activity.

Authors:  T ASTRUP; S MULLERTZ
Journal:  Arch Biochem Biophys       Date:  1952-10       Impact factor: 4.013

3.  A new acid hydrolysis method for determining tryptophan in peptides and proteins.

Authors:  B Penke; R Ferenczi; K Kovács
Journal:  Anal Biochem       Date:  1974-07       Impact factor: 3.365

4.  Human alpha-2-macroglobulin, characterization and trypsin binding. Purification methods, trypsin and plasmin complex formation.

Authors:  U Hamberg; P Stelwagen; H S Ervast
Journal:  Eur J Biochem       Date:  1973-12-17

5.  On fibrinolysis. A. In pregnancy, labour, puerperium and during treatment with sex hormones. B. In human ontogenesis and in human organ culture.

Authors:  B Astedt
Journal:  Acta Obstet Gynecol Scand Suppl       Date:  1972

6.  Measurement of pregnancy associated plasma protein A (PAPP-A).

Authors:  P Bischof; D Bruce; P Cunningham; A Klopper
Journal:  Clin Chim Acta       Date:  1979-07-16       Impact factor: 3.786

7.  The interaction of alpha 2-macroglobulin with proteinases. Characteristics and specificity of the reaction, and a hypothesis concerning its molecular mechanism.

Authors:  A J Barrett; P M Starkey
Journal:  Biochem J       Date:  1973-08       Impact factor: 3.857

8.  Measurement of pregnancy-associated plasma proteins during human gestation.

Authors:  T M Lin; S P Halbert; W N Spellacy
Journal:  J Clin Invest       Date:  1974-09       Impact factor: 14.808

9.  Placental localization of human pregnancy--associated plasma proteins.

Authors:  T M Lin; S P Halbert
Journal:  Science       Date:  1976-09-24       Impact factor: 47.728

10.  Thio reduction of human 2 -macroglobulin. The subunit structure.

Authors:  J M Jones; J M Creeth; R A Kekwick
Journal:  Biochem J       Date:  1972-03       Impact factor: 3.857
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  7 in total

1.  Binding of pregnancy-associated plasma protein-A (PAPP-A) to placental subfractions.

Authors:  K Isaka; P Bischof
Journal:  Arch Gynecol       Date:  1986

2.  The disappearance rate of pregnancy-associated plasma protein-A (PAPP-A) after the end of normal and abnormal pregnancies.

Authors:  P Bischof; M Amandruz; C Weil-Franck; J P Baeriswyl; A Weil; W L Hermann; P C Sizonenko
Journal:  Arch Gynecol       Date:  1984

3.  The development and validation of a radioimmunoassay for human pregnancy-associated plasma protein A (PAPP-A).

Authors:  L G Pinto Furtado; A E Bolton; J G Grudzinskas; M G Chapman; M J Sinosich; V Sharma
Journal:  Arch Gynecol       Date:  1984

4.  Studies on human pregnancy-associated plasma protein A. Purification by affinity chromatography and structural comparisons with alpha 2-macroglobulin.

Authors:  R G Sutcliffe; B M Kukulska-Langlands; J R Coggins; J B Hunter; C H Gore
Journal:  Biochem J       Date:  1980-12-01       Impact factor: 3.857

5.  Immunological studies of the human placenta: functional and morphological analysis of pregnancy-associated plasma protein A (PAPP-A).

Authors:  J A McIntyre; B Hsi; W P Faulk; A Klopper; R Thomson
Journal:  Immunology       Date:  1981-11       Impact factor: 7.397

6.  Enzyme immunometric assay for the determination of pregnancy associated plasma protein A (PAPP-A) with the antigen as solid phase (conjoint IEMA).

Authors:  N A Bersinger
Journal:  Experientia       Date:  1984-09-15

7.  PAPPA-mediated adipose tissue remodeling mitigates insulin resistance and protects against gestational diabetes in mice and humans.

Authors:  Raziel Rojas-Rodriguez; Rachel Ziegler; Tiffany DeSouza; Sana Majid; Aylin S Madore; Nili Amir; Veronica A Pace; Daniel Nachreiner; David Alfego; Jomol Mathew; Katherine Leung; Tiffany A Moore Simas; Silvia Corvera
Journal:  Sci Transl Med       Date:  2020-11-25       Impact factor: 17.956
  7 in total

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