Literature DB >> 6084475

The development and validation of a radioimmunoassay for human pregnancy-associated plasma protein A (PAPP-A).

L G Pinto Furtado, A E Bolton, J G Grudzinskas, M G Chapman, M J Sinosich, V Sharma.   

Abstract

A specific and sensitive radioimmunoassay for human pregnancy-associated plasma protein A (PAPP-A) is described. This assay utilized tracer repurified by affinity chromatography on heparin-Sepharose. The most sensitive assay developed had a detection limit of 5 microliters PAPP-A in serum, and this protein could not be detected in sera from non-pregnant subjects. During pregnancy, mean circulating levels rose from 0.6 mg/l at 6 weeks gestation to 110 mg/l at term, and PAPP-A was detected in 44 of 101 umbilical blood samples. The use of the anticoagulants EDTA and acid citrate dextrose resulted in significantly different results in the plasma compared to the corresponding serum sample. PAPP-A was also measured in amniotic fluid.

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Year:  1984        PMID： 6084475     DOI： 10.1007/bf02134004

Source DB:  PubMed          Journal:  Arch Gynecol        ISSN： 0170-9925


  19 in total

1.  Preparation of iodine-131 labelled human growth hormone of high specific activity.

Authors:  W M HUNTER; F C GREENWOOD
Journal:  Nature       Date:  1962-05-05       Impact factor: 49.962

2.  The radioimmunoassay of placental protein 5 and circulating levels in maternal blood in the third trimester of normal pregnancy.

Authors:  B Obiekwe; D J Pendlebury; Y B Gordeon; J G Grudzinskas; T Chard; H Bohn
Journal:  Clin Chim Acta       Date:  1979-08-01       Impact factor: 3.786

3.  Radioimmunoassay for the measurement of pregnancy-associated plasma protein-A (PAPP-A) in humans.

Authors:  P Bischof; L Haenggeli; M T Sizonenko; W Herrmann; P C Sizonenko
Journal:  Biol Reprod       Date:  1981-06       Impact factor: 4.285

4.  Radioimmunoassay for pregnancy-associated plasma protein A.

Authors:  M J Sinosich; B Teisner; J Folkersen; D M Saunders; J G Grudzinskas
Journal:  Clin Chem       Date:  1982-01       Impact factor: 8.327

5.  Specific and reversible interaction between pregnancy-associated plasma protein A and heparin.

Authors:  J G Westergaard; J Hau; B Teisner; J G Grudzinskas
Journal:  Placenta       Date:  1983 Jan-Apr       Impact factor: 3.481

6.  Interaction between heparin and human pregnancy-associated plasma protein A (PAPP-A): a simple purification procedure.

Authors:  M W Davey; B Teisner; M Sinosich; J G Grudzinskas
Journal:  Anal Biochem       Date:  1983-05       Impact factor: 3.365

7.  Pregnancy-associated plasma protein A in the prediction of early pregnancy failure.

Authors:  J G Westergaard; M J Sinosich; M Bugge; L T Madsen; B Teisner; J G Grudzinskas
Journal:  Am J Obstet Gynecol       Date:  1983-01-01       Impact factor: 8.661

8.  Development of a radioimmunoassay for pregnancy-associated plasma protein A and establishment of normal levels in the first trimester of pregnancy.

Authors:  F Anthony; G M Masson; P J Wood
Journal:  Ann Clin Biochem       Date:  1983-01       Impact factor: 2.057

9.  Circulating levels of pregnancy-associated plasma protein-A (PAPP-A) and human chorionic gonadotrophin (hCG) in intrauterine and extrauterine pregnancies.

Authors:  P Bischof; H Reyes; W L Herrmann; P C Sizonenko
Journal:  Br J Obstet Gynaecol       Date:  1983-04

10.  Purification and characterization of pregnancy associated plasma protein A (PAPP-A).

Authors:  P Bischof
Journal:  Arch Gynecol       Date:  1979
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  1 in total

1.  A sensitive enzyme immunoassay for pregnancy-associated plasma protein A (PAPP-A): a possible first trimester method of screening for Down syndrome and other trisomies.

Authors:  N A Bersinger; A Zakher; U Huber; G Pescia; H Schneider
Journal:  Arch Gynecol Obstet       Date:  1995       Impact factor: 2.344
  1 in total

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