Literature DB >> 4965915

Detection of botulinal toxins by immunodiffusion.

B L Vermilyea, H W Walker, J C Ayres.   

Abstract

A procedure employing concentration with Sephadex and analysis by gel diffusion (Ouchterlony) was used to detect the toxins of Clostridium botulinum in foods. Botulinal toxins with toxic levels of 370 to 557 mouse LD(50) per milliliter were detected in the food samples. Test results were verified by use of the mouse protection test. Approximately 24 hr were required to complete the entire procedure.

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Year:  1968        PMID: 4965915      PMCID: PMC547297          DOI: 10.1128/am.16.1.21-24.1968

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN: 0003-6919


  8 in total

1.  ASSAY OF STAPHYLOCOCCAL ENTEROTOXIN FROM CHEESE.

Authors:  R B READ; J BRADSHAW; W L PRITCHARD; L A BLACK
Journal:  J Dairy Sci       Date:  1965-04       Impact factor: 4.034

2.  DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN IN FOOD.

Authors:  E P CASMAN; R W BENNETT
Journal:  Appl Microbiol       Date:  1965-03

3.  A simplified micro double-diffusion agar precipitin technique.

Authors:  A J CROWLE
Journal:  J Lab Clin Med       Date:  1958-11

4.  Studies on immunity to toxins of Clostridium botulinum. III. Preparation, purification, and detoxification of type E toxin.

Authors:  M GORDON; M A FIOCK; A YARINSKY; J T DUFF
Journal:  J Bacteriol       Date:  1957-10       Impact factor: 3.490

5.  A slide microtechnique for the analysis of immune precipitates in gel.

Authors:  C WADSWORTH
Journal:  Int Arch Allergy Appl Immunol       Date:  1957

6.  Study of toxins of Clostridium botulinum. III. Relation of autolysis to toxin production.

Authors:  D A BOROFF
Journal:  J Bacteriol       Date:  1955-10       Impact factor: 3.490

7.  Serological studies of types A, B, and E botulinal toxins by passive hemagglutination and bentonite flocculation.

Authors:  H M Johnson; K Brenner; R Angelotti; H E Hall
Journal:  J Bacteriol       Date:  1966-03       Impact factor: 3.490

8.  PREPARATION AND ASSAY OF THE INTERNATIONAL STANDARDS FOR CLOSTRIDIUM BOTULINUM TYPES A, B, C, D AND E ANTITOXINS.

Authors:  E J BOWMER
Journal:  Bull World Health Organ       Date:  1963       Impact factor: 9.408

  8 in total
  11 in total

1.  Rapid gas chromatographic technique for presumptive detection of Clostridium botulinum in contaminated food.

Authors:  J W Mayhew; S L Gorbach
Journal:  Appl Microbiol       Date:  1975-02

2.  Monoclonal antibody to type F Clostridium botulinum toxin.

Authors:  J L Ferreira; M K Hamdy; S G McCay; F A Zapatka
Journal:  Appl Environ Microbiol       Date:  1990-03       Impact factor: 4.792

3.  Evaluation of type A botulinal toxin assays that use antitoxin to crystalline toxin.

Authors:  H Sugiyama; I Oishi; B R Dasgupta
Journal:  Appl Microbiol       Date:  1974-02

4.  Rapid detection of Clostridium botulinum toxin by capillary tube diffusion.

Authors:  L W Mestrandrea
Journal:  Appl Microbiol       Date:  1974-06

5.  Rapid detection and quantitative estimation of type A botulinum toxin by electroimmunodiffusion.

Authors:  C A Miller; A W Anderson
Journal:  Infect Immun       Date:  1971-08       Impact factor: 3.441

Review 6.  Laboratory diagnostics of botulism.

Authors:  Miia Lindström; Hannu Korkeala
Journal:  Clin Microbiol Rev       Date:  2006-04       Impact factor: 26.132

7.  Immunodiffusion method for detection of type A Clostridium botulinum.

Authors:  J L Ferreira; M K Hamdy; F A Zapatka; W O Hebert
Journal:  Appl Environ Microbiol       Date:  1981-12       Impact factor: 4.792

8.  Noncorrelation between mouse toxicity and serologically assayed toxin in Clostridium botulinum type A culture fluids.

Authors:  M J Betley; H Sugiyama
Journal:  Appl Environ Microbiol       Date:  1979-08       Impact factor: 4.792

9.  Sensing the deadliest toxin: technologies for botulinum neurotoxin detection.

Authors:  Petr Capek; Tobin J Dickerson
Journal:  Toxins (Basel)       Date:  2010-01-07       Impact factor: 4.546

10.  Novel Mutant Phospholipase D from Hemiscorpius lepturus Acts as A Highly Immunogen in BALB/c Mice Against the Lethality of Scorpion Venom.

Authors:  Abouzar Soleimani Moez; Reza H Sajedi; Kamran Pooshang Bagheri; Jean-Marc Sabatier; Delavar Shahbazzadeh
Journal:  Molecules       Date:  2020-04-04       Impact factor: 4.411

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